WEBINAR: Measuring the Growth of Microbial Biofilms in Real-Time: Evidence, Insights and Applications

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WEBINAR: Measuring the Growth of Microbial Biofilms in Real-Time: Evidence, Insights and Applications

Alex Mira, Ph.D.
Center for Advanced Research in Public Health, FISABIO Foundation, Valencia (Spain)

In this webinar, we will:

  1. Provide an overview of how impedance-monitoring by xCELLigence instruments can be used to track the dynamics (attachment, growth, dissipation) of bacterial and fungal biofilms
  2. Validate the utility of the xCELLigence assay by comparing WT vs. mutant strains that are deficient in biofilm production, and by comparing the xCELLigence impedance signal with standard quantification methods such as safranin staining
  3. Analyze both single- and multi-species biofilms, with the goal of using xCELLigence plates as an in vitro model for complex human-associated biofilms
  4. Demonstrate the use of xCELLigence for anti-biofilm drug screening and antibiotic susceptibility testing, with comparison to the results obtained by traditional methods such as epsilon-tests
  5. Look at efforts to employ xCELLigence as a clinical theranostic tool for identifying the optimal treatment regimen for biofilm infections

About the speaker
Alex Mira obtained his Ph.D. in microbiology at Oxford University (UK) in 1999, and carried out post-doctoral research in the USA and Sweden working on microarray technology, microbial genomics, and bioinformatics. In 2003 he started his own research group studying oral microbiota using ‘omics’ techniques. In 2009, he was awarded the “Jaime Ferran” Spanish Award for Research in Microbiology. He is currently the principal investigator of the Oral Microbiome Laboratory at the CSISP, in Spain, where he has applied metagenomics, next-generation sequencing, and impedance technology to study the human microbiome and human-associated biofilms. His team has helped to pioneer use of the xCELLigence instrument for studying microbial biofilms, including applications such as: 1) discovering drugs that inhibit biofilm growth, 2) measuring antibiotic susceptibility in biofilm-mediated infections (i.e. clinical theranostics), and 3) developing an in vitro model of oral diseases.