Induced pluripotent stem cells (iPSCs) have made it possible to establish patient-derived cell lines that recapitulate the specific disease phenotypes seen in the human donor. In this video Dr. Jason Maynes, Principle Investigator at The Hospital for Sick Children, describes using the xCELLigence CardioECR system to simultaneously analyze the function (contractility) and ion channel activity of diseased pediatric iPSC cardiomyocytes. This footage of Dr. Maynes’ is from a recent episode of the Discovery Channel’s “Innovations” TV program (aired December 18th, 2014). The complete program can be viewed here: https://www.youtube.com/watch?v=44bpQT3lCnk
Jason T. Maynes, M.D., Ph.D.,
Director of Anesthesia and Pain Medicine Research
The Hospital for Sick Children
Dr. Jason Maynes currently serves as Staff Anesthesiologist, Director of Anesthesia and Pain Medicine Research and Principal Investigator at the Hospital for Sick Children. His lab’s focus combines his clinical and research interests of assessing the mechanism of anaesthetic action and anaesthetic off-targets and elucidating the role of proteins in mitochondrial dynamics. Dr. Maynes’ group specializes in high-content imaging to assay mitochondria and other cellular processes. They also apply these techniques to study other disease phenotypes including, dilated cardiomyopathy and chemotherapy-induced cardiac dysfunction. Using custom assays, they further conduct high-throughput drug screening to identify agents that attenuate or eliminate such disease phenotypes. Dr. Maynes received his MD and PhD degrees from the University of Alberta and conducted his clinical training in paediatrics and anaesthesia at Barnes-Jewish Hospital/St. Louis Children’s/Washington University in St. Louis while concurrently pursuing a post-doctoral fellowship in biophysics at the Los Alamos National Laboratory, Washington University.
Traister A, Li M, Aafaqi S, Lu M, Arab S, Radisic M, Gross G, Guido F, Sherret J, Verma S, Slorach C, Mertens L, Hui W, Roy A, Delgado-Olguín P, Hannigan G, Maynes JT, Coles JG. Integrin-linked kinase mediates force transduction in cardiomyocytes by modulating SERCA2a/PLN function. Nat Commun. 2014 Sep 11;5:4533.