Advances in Toxicity Bioassays – Unleashing the Power of Real-Time Cell Analysis for Health Risk Assessment

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Stephan Gabos photoDr. Stephan Gabos

University of Alberta, Edmonton, AB (Canada)

Modern public health protection requires ongoing surveillance of environmental media (water, air, food and soil) for potentially harmful chemicals. There is currently an unmet need for in vitro toxicity assays with higher sensitivity, lower interference, and better predictive value than what is possible using animal studies. Dr. Stephan Gabos at the University of Alberta in Canada has been developing such assays for the detection and evaluation of environmental chemicals using the xCELLigence® Real-Time Cell Analysis (RTCA) technology. During this webinar, Dr. Gabos addresses:

  • The health risk assessment paradigm of the 21st century
  • Current public/environmental toxicology applications including: classification of chemicals into the Globally Harmonized System (GHS) categories, prediction of a compound’s mode of action (MOA),water toxicity testing, assays for nanoparticles and air particulate matter, and identification of mutagenic substances
  • Future trends in public health toxicology and health risk assessment

Dr. Stephan Gabos is currently a research scientist within the Division of Analytical and Environmental Toxicology in the Department of Laboratory Medicine and Pathology at the University of Alberta. Prior to this, he was the senior science advisor within the Office of the Chief Medical Officer of Health (Alberta, Canada). Dr. Gabos received his M.D. from the University of Medicine and Pharmacy, Tirgu Mures, Romania in 1976, and research training in pathology and epidemiology at the University of Medicine and Pharmacy, Tirgu Mures, Tufts University, and the John Hopkins Hospital.  He has a broad range of research interests including environmental toxicology and epidemiology, cytotoxicity, exposure assessment, biomonitoring, and health risk assessment. He has published over 50 papers in peer reviewed journals, and has authored several cytology and histology manuals and book chapters. He has been employed in a variety of research, administrative, and executive management positions within the Cancer Board and the Ministry of Health in Alberta, Canada. His former positions include Head of Environmental Epidemiology and Director of Health Surveillance, Alberta Health, Edmonton. For his service to the government of Alberta and his contributions in the field of environmental health, Dr. Gabos received the Premier’s Award of Excellence four separate times.

REFERENCES:

  1. Xing JZ, Zhu L, Jackson JA, Gabos S, Sun XJ, Wang XB, Xu X. Dynamic monitoring of cytotoxicity on microelectronic sensors. Chem Res Toxicol. 2005 Feb;18(2):154-61.
  2. Moe, B., McGuigan, C. F., Li, X.-F., Dabek-Zlotorzynska, E. and Gabos, S. 2014. Cell-Electronic Sensing of Cellular Responses to Micro- and Nanoparticles for Environmental Applications. Encyclopedia of Analytical Chemistry. 1–28.
  3. Ibrahim F, Huang B, Xing J, Gabos S. Early determination of toxicant concentration in water supply using MHE. Water Res. 2010 May;44(10):3252-60.
  4. Pan T, Huang B, Zhang W, Gabos S, Huang DY, Devendran V. Cytotoxicity assessment based on the AUC50 using multi-concentration time-dependent cellular response curves. Anal Chim Acta. 2013 Feb 18;764:44-52.
  5. Pan T, Khare S, Ackah F, Huang B, Zhang W, Gabos S, Jin C, Stampfl M. In vitro cytotoxicity assessment based on KC(50) with real-time cell analyzer (RTCA) assay. Comput Biol Chem. 2013 Dec;47:113-20.
  6. Pan TH, Huang B, Xing JZ, Zhang WP, Gabos S, Chen J. Recognition of chemical compounds in contaminated water using time-dependent multiple dose cellular responses. Anal Chim Acta. 2012 Apr 29;724:30-9.
  7. Huang DY, Mock M, Hagenbuch B, Chan S, Dmitrovic J, Gabos S, Kinniburgh D. Dynamic cytotoxic response to microcystins using microelectronic sensor arrays. Environ Sci Technol. 2009 Oct 15;43(20):7803-9..
  8. Boyd JM, Huang L, Xie L, Moe B, Gabos S, Li XF. A cell-microelectronic sensing technique for profiling cytotoxicity of chemicals. Anal Chim Acta. 2008 May 12;615(1):80-7.
  9. Moe B, Gabos S, Li XF. Real-time cell-microelectronic sensing of nanoparticle-induced cytotoxic effects. Anal Chim Acta. 2013 Jul 30;789:83-90.
  10. Xing JZ, Gabos S, Huang B, Pan T, Huang M, Chen J. High-throughput quantitative analysis with cell growth kinetic curves for low copy number mutant cells. Anal Bioanal Chem. 2012 Oct;404(6-7):2033-41.
  11. Pan T, Li H, Khare S, Huang B, Yu Huang D, Zhang W, Gabos S. High-throughput screening assay for the environmental water samples using cellular response profiles. Ecotoxicol Environ Saf. 2015 Apr;114:134-42.