A Novel in vitro Approach to Study Biocompatibility and Wound Healing


Sandra Garcia photoDr. Sandra N. Garcia

Kinetic Concepts Inc., San Antonio, TX

Negative Pressure Wound Therapy (NPWT), where a vacuum is applied to an acute or chronic wound, has proven extremely effective for promoting would healing.  The success of NPWT lies in its ability to draw the edges of a wound together, to promote granulation, and to remove infectious material.  Critical to NPWT’s efficacy was the development of biocompatible materials capable of interfacing with the wound throughout the healing process. In this webinar Dr. Sandra N. Garcia from Kinetic Concepts Inc. (San Antonio, TX) describes the development of in vitro assays used to evaluate medical devices for toxicity/biocompatibility and efficacy in promoting wound healing. Specific topics addressed include:

  • Comparison of xCELLigence® real-time cellular analysis vs. conventional methods (visual assessment or WST-1 as described in ISO 10933-5) for assessing the cytotoxicity of medical devices in vitro
  • Advantages of the xCELLigence® cell invasion/migration (CIM) assay vs. a standard scratch assay for studying wound healing
  • Application of the xCELLigence® cell invasion/migration (CIM) device for evaluating the efficacy of Negative Pressure Wound Therapy (V.A.C.® NPWT)

Dr. Sandra N. Garcia is a cell and tissue culture manager at Kinetic Concepts Inc. (KCI), a global medical device leader in transformational wound healing solutions headquartered in San Antonio TX. Dr. Garcia attended the University of Texas, San Antonio, and subsequently received her Ph.D. in cellular and molecular biology from the University of California, San Diego. She has over 20 years of experience as a research scientist, including two post-doctoral fellowships where she focused on cellular senescence and gene silencing at the Sam and Ann Barshop Institute and the University of Texas, San Antonio. More recently, Dr. Garcia has directed research elucidating the mechanisms of action for: CelluTome® (an automated epidermal micrografting tool), Grafjacket® (an acellular matrix), and V.A.C™ therapy on multiple patient stem cell populations. For more information about KCI or on-going studies, visit www.kci1.com or contact Dr. Garcia at sandra.garcia@kci1.com.


Garcia SN, Gutierrez L, McNulty A. Real-time cellular analysis as a novel approach for in vitro cytotoxicity testing of medical device extracts. J Biomed Mater Res A. 2013 Jul;101(7):2097-106