NovoCyte Support

NovoCyte Support


NovoCyte Instrument

Any cell or particle in size from 0.2-50 µm can be assayed on the system.

The NovoCyte is available in four different models which have 1, 2, or 3 lasers and from 3 to 13 fluorescence channels.

Innovations in the optical layout, electronic signal processing and the obscuration bar design result in high sensitivity and resolution for both scattered light signals and fluorescence signals:
Forward/side scatter resolution: FSC: 0.5 µm; SSC: 0.2 µm
Fluorescence sensitivity: FITC < 75 MESF; PE < 50 MESF

<3% CV for chicken erythrocyte nuclei DNA content analysis.

The sample flow rate can be adjusted continuously in the range of 5-120 µL/min. The maximum sample processing rate is 35,000 events per second.

The flow core diameter can be varied 4.6 to 22.7µm, corresponding to the flow rate range of 5 to 120 µL/min. The software indicates the altered flow core diameter as you modify the flow rate.

The NovoCyte is able to record up to 10 million events (either total or within a gate of interest), up to 5 mL of sample volume, and/or up to 120 min for each sample acquisition.

The syringe pump aspirates from 10 to 100 µL for each sample acquired plus 20 µL of void volume.

The NovoCyte uses advanced electronics and data processing algorithms to provide 7 decades of dynamic range. This high dynamic range eliminates the need for laborious PMT adjustment before each experiment.

Startup and shutdown cleaning routines are performed automatically, eliminating the need for time-consuming manual cleaning or decontamination protocols.
Also, the NovoCyte utilizes sensors in the fluidic pathway to monitor the system status in real time. Abnormal working conditions are reported on the NovoExpress software and one-click automated procedures can be executed, including routines that will backflush, debubble, unclog, prime, rinse, or clean the fluidics system.
Finally, fluidics system filters and tubing components may easily be changed on a recommended schedule (bi-monthly or bi-yearly, depending on the component) using kits available from ACEA.

No. The NovoCyte can do direct absolute counting with no need for expensive reference counting beads. The high precision syringe pump guarantees an accurate volume of sample is dispensed.

The precision of the direct absolute counting is < 5% CV.

The sample injection probe (SIP) is washed automatically after each sample collection, minimizing the possibility of sample carryover (<0.1%).

All of the necessary reagents to QC and run the instrument are available from ACEA, including NovoCyte Instrument QC Particles, NovoFlow sheath fluid, NovoRinse solution and NovoClean solution.

The sheath fluid consumption rate is 6.25 mL/min.

• NovoCyte: W × D × H: 60 × 45 × 39 cm (23.6 × 17.7 × 15.4 in.)
• Fluidics Station (including reagent containers): W × D × H: 25 × 29 × 42 cm (10.0 ×11.4 × 16.5 in.)
• NovoSampler: W × D × H: 31 × 26 × 28 cm (12.2 ×10.2 × 11.0 in.)

Instrument QC

The NovoExpress software includes an automatic QC function. Just prepare the QC beads and click the QC test button on the NovoExpress software. A QC report is automatically generated and the results are displayed after about 2 minutes. A Levey-Jennings plot for the mean and CV of specific parameters is provided to track the performance of the instrument over time. This simple procedure may be done on a daily basis.

NovoExpress Software

The NovoExpress software contains all the tools you expect for collecting and analyzing your flow data, including a variety of plot types (dot, density [grey or pseudo-color], contour, and histograms). In addition, a drag-and-drop software templating function allows for rapid application of settings and analysis across multiple samples. A built-in cell cycle analysis function (Watson pragmatic model with customizable constraints) eliminates the need for third party software.

The NovoCyte is a digital system, allowing for compensation after data acquisition. The NovoExpress software allows for direct editing of the compensation matrix as well as a simple-to-use Quick Compensation sliding bar to quickly achieve proper compensation. Also, NovoExpress provides an Auto Compensation function: after collection of data for single-stained samples, the software automatically calculates the compensation matrix for you.

The NovoExpress software generates .ncf files by default. It includes the data, compensation matrix, acquisition settings and analysis templates.

Yes, NovoCyte data may be exported in FSC3.0 format for analysis on third party software such as FlowJo. Also, NovoCyte data may be exported as a CSV file for analysis with programs such as Microsoft Excel.

Yes, the NovoExpress software is able to import and analyze FCS3.0 files.

Yes.  Simply right click on a dot plot/histogram, select “edit overlays”, and then choose the sample or gate of interest.

Yes, the NovoExpress software is provided with a cell cycle module based on the Watson pragmatic model. To use it, just click on the corresponding icon on the tool bar.

Autosampler / NovoSampler

The optional NovoSampler allows for use of standard 24- or 96-well microtiter plates (U/V/flat-bottom) as well as a 24 tube rack (provided by ACEA) for use with standard 12X75 mm tubes. The modular design of the NovoCyte autosampler allows for convenient installation and calibration without the need for special tools.

A 96 well plate can be processed automatically in less than 60 minutes, using a 10 uL acquisition volume from each well.

Yes. Mixing is performed by agitating the plate using customizable, user-defined cycles.

A user-defined rinse program (0-3 cycles) for cleaning the fluidic system and the sample injection probe ensures that carryover is negligible (<0.1% with 1 rinse cycle per well).

Application Notes

Spec Sheets

Videos: Product Overviews

Videos: Research Presentations

Live Technical Webinar: Compensation in Flow Cytometry - Date: June 28, 2018 About this Webinar Compensation in flow cytometry can often be challenging and sometimes confusing, however doing compensation correctly is absolutely essential for valid and optimal analyses of multicolor flow cytometry experiments. This webinar will discuss fluorescence spillover, compensation, how to calculate compensation accurately, best practices and considerations for control samples, and some … Continued

Date: June 28, 2018

About this Webinar

Compensation in flow cytometry can often be challenging and sometimes confusing, however doing compensation correctly is absolutely essential for valid and optimal analyses of multicolor flow cytometry experiments. This webinar will discuss fluorescence spillover, compensation, how to calculate compensation accurately, best practices and considerations for control samples, and some challenges that may be encountered. Participants will learn tips and tricks on how to prepare high-quality compensation controls and how to create an optimal compensation matrix, resulting in higher confidence in data analyses and more robust data.

About the Presenter

Heather Paich, Ph.D.
Field Application Scientist
ACEA Biosciences Inc.

Heather Paich received her PhD in Nutritional Biochemistry at the University of North Carolina at Chapel Hill where her research focused on how obesity affects the immune response to pandemic H1N1 influenza. After completing a Fogarty Global Health Post-Doctoral Fellowship in Cape Town, South Africa, where her work focused on infectious diseases, she joined ACEA Biosciences, where she is currently a Senior Field Application Scientist for Flow Cytometry.

A Flow Cytometry Method for Detecting Bacteria in Water - About this Webinar Regulatory testing for microbiological water quality is often conducted by growing microbes on agar medium in visible colonies. However, this is a time-consuming process and not all bacteria can grow in media and therefore be detected using this method. Flow cytometers with a high sensitivity of detection provide tools for detecting and … Continued

About this Webinar

Regulatory testing for microbiological water quality is often conducted by growing microbes on agar medium in visible colonies. However, this is a time-consuming process and not all bacteria can grow in media and therefore be detected using this method. Flow cytometers with a high sensitivity of detection provide tools for detecting and analysing microbes independent of their cultivability and allows precise and rapid determinations of microbial bulk parameters and delivers detailed information on the general microbial state.

During this webinar, we will cover:

1. Advantages of flow cytometry for microbiological water quality testing
2. How to determine absolute counts, nucleic acid content, activity, and classification of bacteria
3. Tips on running a flow cytometry experiment for microbial analysis
4. Data analysis of treated water samples using the NovoExpress software

 

About the Presenter

Stephanie Ting, M. Eng
Field Application Scientist – SE Asia, ANZ, HK&Macau, India,
ACEA Biosciences Inc.

Stephanie received her Masters in Mechanical Engineering from the University of Tokyo, Japan where she studied the effects of mechanobiological stimuli on the production of cartilage-like tissues using mesenchymal stem cells. Currently, she is the Field Application Scientist for SE Asia, ANZ, HK&Macau, and India.

 

WEBINAR – Cell Cycle Analysis by Flow Cytometry -  About this Webinar DNA content analysis using flow cytometry is a powerful tool that can determine the effect of treatments on cell cycle and ploidy in particular relevance to tumours. However, how do you determine which DNA-binding dye to use? How do you accurately differentiate the different phases in the cell cycle in your … Continued

About this Webinar

DNA content analysis using flow cytometry is a powerful tool that can determine the effect of treatments on cell cycle and ploidy in particular relevance to tumours. However, how do you determine which DNA-binding dye to use? How do you accurately differentiate the different phases in the cell cycle in your population of cells? This webinar will teach the methods for cell cycle analysis with the following topics:

During this webinar, we will cover:

  • Phases of a typical mammalian cell cycle
  • Characteristics of Nucleic Acid Stains
  • How to choose the right fluorochromes and properly prepare your cells
  • DNA ploidy & tumours

Session One:

Date: Thursday, December 21, 2017
Time: 8 AM SINGAPORE | 9 AM TOKYO | 4 PM PST (Dec. 20)

Session Two:

Date: Thursday, December 21, 2017
Time: 7 AM PST | 10 AM EST | 4 PM PARIS

 

About the Presenter

Stephanie Ting, M. Eng
Field Application Scientist – SE Asia, ANZ, HK&Macau, India,
ACEA Biosciences Inc.

Stephanie received her Masters in Mechanical Engineering from the University of Tokyo, Japan where she studied the effects of mechanobiological stimuli on the production of cartilage-like tissues using mesenchymal stem cells. Currently, she is the Field Application Scientist for SE Asia, ANZ, HK&Macau, and India.

 

 

WEBINAR – Measuring Calcium Flux by Flow Cytometry - Accurate measurements of intracellular Ca2+ concentrations allows for a more comprehensive understanding of Ca2+ regulated cell functions and signaling pathways. Ca2+ is an important molecule that affects a wide range of physiological and pathological processes.

Accurate measurements of intracellular Ca2+ concentrations allows for a more comprehensive understanding of Ca2+ regulated cell functions and signaling pathways. Ca2+ is an important molecule that affects a wide range of physiological and pathological processes. Unlike other secondary messengers, Ca2+ is not synthesized or metabolized, but stored and rapidly released by channels/pumps that maintain Ca2+ concentrations in distinct cellular compartments. Flow cytometry allows real time measurement of a calcium flux response utilizing fluorescent Ca2+ indicators which exhibit an increase in fluorescence upon binding to Ca2+. In this webinar we will discuss the basics of calcium flux measurements by flow cytometry, providing specific details on experiment setup, acquisition, and analysis on the NovoCyte flow cytometer.

Key topics to be covered:
1. Essentials of calcium measurements by flow cytometer
2. Calcium flux experimental setup and acquisition on the NovoCyte
3. Detailed analysis of calcium flux in a T cell line using calcium indicator dye Fluo-4/AM

Learn More about the NovoCyte Flow Cytometer       Click here

Speaker:

Lauren Jachimowicz, Ph.D. 
Application Development Scientist, ACEA Biosciences, Inc.
Lauren Jachimowicz received her PhD in Biological Science from the University of California, San Diego where she studied the role of the circadian clock genes in T cells. Currently, she is the Application Development Scientist for flow cytometry at ACEA Biosciences advancing flow cytometry into new fields of research.

WEBINAR: Flow Cytometric Assessment, Quantification and Regulation of Human Neutrophil Extracellular Traps (NETS) - At the CYTO 2017 meeting Dr. Adriano Rossi and Dr. Calum Robb from the University of Edinburgh described the development of a new flow cytometry-based assay for studying NETosis, a cell death process that occurs when stimulated neutrophils cast an extracellular web containing DNA, histones, and anti-bacterial proteins that ensnare/neutralize invading organisms. Using the NovoCyte® … Continued


At the CYTO 2017 meeting Dr. Adriano Rossi and Dr. Calum Robb from the University of Edinburgh described the development of a new flow cytometry-based assay for studying NETosis, a cell death process that occurs when stimulated neutrophils cast an extracellular web containing DNA, histones, and anti-bacterial proteins that ensnare/neutralize invading organisms. Using the NovoCyte® flow cytometer, they demonstrate the detection and quantification of neutrophil extracellular traps (NETs), as well as analysis of the regulatory mechanisms involved.

WEBINAR: Flow Cytometry: Simplifying Assay Panel Design and WorkFLOW - About this Webinar Researchers and flow cytometry users spend a significant amount of time designing flow assay panels and analyzing their samples. In this webinar, viewers will have the chance to learn about  tools and recent developments in flow cytometry  that simplify assay design and workflow. Our speakers will explore the capabilities of benchtop and … Continued

About this Webinar

Researchers and flow cytometry users spend a significant amount of time designing flow assay panels and analyzing their samples.

In this webinar, viewers will have the chance to learn about  tools and recent developments in flow cytometry  that simplify assay design and workflow. Our speakers will explore the capabilities of benchtop and cost-effective flow cytometry systems and the easiest way to design flow panels.

Webinar Features

  • Design and capabilities of a NovoCyte flow cytometer
  • New features that simplify setup, acquisition, and analysis
  • How to design multicolor fluorescent panels using FluoroFinder’s experiment design platform
  • Tips for building better panels in less time

Featured Speakers:

Garret Guenther, Ph.D.
ACEA Biosciences.

Jeff Jensen, Ph.D.
FluoroFinder, LLC

 

NovoCyte Webinar Series – Measuring Apoptosis by Flow Cytometry - Apoptosis, or programmed cell death, is a tightly regulated cellular phenomenon which includes many different signaling and effector pathways. By interrogating these processes in individual cells, and by evaluating large numbers of cells rapidly, flow cytometry can efficiently

WEBINAR – Flow Cytometry: Emerging Instrumentation and Application Trends | Wed, Feb 15, 2017 - Flow cytometry remains an essential tool for cell analysis. Although often relegated to specialized core facilities, flow cytometry instruments are in fact becoming increasingly commonplace in life science labs. In this special webinar, viewers will have the chance to learn about recent developments in flow cytometry technology, including the emergence of lower cost, smaller flow cytometry options with the capability of analyzing multiple … Continued

Flow cytometry remains an essential tool for cell analysis. Although often relegated to specialized core facilities, flow cytometry instruments are in fact becoming increasingly commonplace in life science labs.

In this special webinar, viewers will have the chance to learn about recent developments in flow cytometry technology, including the emergence of lower cost, smaller flow cytometry options with the capability of analyzing multiple fluorescent colors. Our speakers will explore the design, development, and uses for smaller, more cost-effective flow cytometry systems in research labs. In this webinar, you will gain insights on:

  • Design and development of the latest multicolor flow cytometry instruments
  • Application of flow cytometry techniques for different experimental needs
  • How analyzing multiple cellular parameters using different  fluorescent colors can extend experimental possibilities
  • How to effectively analyze and interpret your flow cytometry data sets
Learn More about the NovoCyte Flow Cytometer       Click here

Speakers:

Paul K. Wallace, PhD
Roswell Park Cancer Institute
Paul Wallace has served since 2003 as Director of the Flow and Image Cytometry Department at the Roswell Park Cancer Institute (RPCI) in Buffalo, NY, where he is a Professor of Oncology. He is also an Associate Professor of Biotechnical and Clinical Laboratory Sciences at the University at Buffalo. His primary expertise and board certification is in the flow cytometric diagnosis of hematological malignancies.

Christopher Groves 
MedImmune, Inc. 
Christopher Groves is a Senior Manager at MedImmune Inc., where he heads a keystone technology group  challenged with expanding knowledge of disease mechanisms while also delivering innovative solutions in drug discovery and manufacturing that provide scientists access to cutting-edge technological capability.

William Telford, PhD 
Experimental Transplantation and Immunology Branch, NCI-NIH 
William Telford is the Head of the NCI-NIH Flow Cytometry core facility, which provides state-of-the-art flow cytometry as well as imaging technology and instrumentation to users in the research community.

Moderator:

Patrick C.H. Lo, PhD 
Senior Editor, BioTechniques

Videos: Tutorials

Bench Tip Video: Simplifying Cell Proliferation Studies with Flow Cytometry