QC Lot Files
NovoCyte® QC Particles Lot File
1. Download the QC particles lot file corresponding to the lot number of the QC particles.
2. Save the QC particles lot file to the “\QC\QC Beads” folder in the NovoExpress® installation directory ( the default installation directory for NovoExpressTM is C:\Program Files (x86)\NovoExpress ).
3. Run NovoCyte® QC test.
Note: Please upgrade NovoExpress to 1.0.2 or above version to use the QC particles lot files.(Right click on the corresponding lot number, choose ” save link as “, and save to local)
Accurate measurements of intracellular Ca2+ concentrations allows for a more comprehensive understanding of Ca2+ regulated cell functions and signaling pathways. Ca2+ is an important molecule that affects a wide range of physiological and pathological processes. Unlike other secondary messengers, Ca2+ is not synthesized or metabolized, but stored and rapidly released by channels/pumps that maintain Ca2+ concentrations in distinct cellular compartments. Flow cytometry allows real time measurement of a calcium flux response utilizing fluorescent Ca2+ indicators which exhibit an increase in fluorescence upon binding to Ca2+. In this webinar we will discuss the basics of calcium flux measurements by flow cytometry, providing specific details on experiment setup, acquisition, and analysis on the NovoCyte flow cytometer.
Key topics to be covered:
1. Essentials of calcium measurements by flow cytometer
2. Calcium flux experimental setup and acquisition on the NovoCyte
3. Detailed analysis of calcium flux in a T cell line using calcium indicator dye Fluo-4/AM
Lauren Jachimowicz, Ph.D.
Application Development Scientist, ACEA Biosciences, Inc.
Lauren Jachimowicz received her PhD in Biological Science from the University of California, San Diego where she studied the role of the circadian clock genes in T cells. Currently, she is the Application Development Scientist for flow cytometry at ACEA Biosciences advancing flow cytometry into new fields of research.
At the CYTO 2017 meeting Dr. Adriano Rossi and Dr. Calum Robb from the University of Edinburgh described the development of a new flow cytometry-based assay for studying NETosis, a cell death process that occurs when stimulated neutrophils cast an extracellular web containing DNA, histones, and anti-bacterial proteins that ensnare/neutralize invading organisms. Using the NovoCyte® flow cytometer, they demonstrate the detection and quantification of neutrophil extracellular traps (NETs), as well as analysis of the regulatory mechanisms involved.
About this Webinar
Researchers and flow cytometry users spend a significant amount of time designing flow assay panels and analyzing their samples.
In this webinar, viewers will have the chance to learn about tools and recent developments in flow cytometry that simplify assay design and workflow. Our speakers will explore the capabilities of benchtop and cost-effective flow cytometry systems and the easiest way to design flow panels.
- Design and capabilities of a NovoCyte flow cytometer
- New features that simplify setup, acquisition, and analysis
- How to design multicolor fluorescent panels using FluoroFinder’s experiment design platform
- Tips for building better panels in less time
Garret Guenther, Ph.D.
Jeff Jensen, Ph.D.
Flow cytometry remains an essential tool for cell analysis. Although often relegated to specialized core facilities, flow cytometry instruments are in fact becoming increasingly commonplace in life science labs.
In this special webinar, viewers will have the chance to learn about recent developments in flow cytometry technology, including the emergence of lower cost, smaller flow cytometry options with the capability of analyzing multiple fluorescent colors. Our speakers will explore the design, development, and uses for smaller, more cost-effective flow cytometry systems in research labs. In this webinar, you will gain insights on:
- Design and development of the latest multicolor flow cytometry instruments
- Application of flow cytometry techniques for different experimental needs
- How analyzing multiple cellular parameters using different fluorescent colors can extend experimental possibilities
- How to effectively analyze and interpret your flow cytometry data sets
Paul K. Wallace, PhD
Roswell Park Cancer Institute
Paul Wallace has served since 2003 as Director of the Flow and Image Cytometry Department at the Roswell Park Cancer Institute (RPCI) in Buffalo, NY, where he is a Professor of Oncology. He is also an Associate Professor of Biotechnical and Clinical Laboratory Sciences at the University at Buffalo. His primary expertise and board certification is in the flow cytometric diagnosis of hematological malignancies.
Christopher Groves is a Senior Manager at MedImmune Inc., where he heads a keystone technology group challenged with expanding knowledge of disease mechanisms while also delivering innovative solutions in drug discovery and manufacturing that provide scientists access to cutting-edge technological capability.
William Telford, PhD
Experimental Transplantation and Immunology Branch, NCI-NIH
William Telford is the Head of the NCI-NIH Flow Cytometry core facility, which provides state-of-the-art flow cytometry as well as imaging technology and instrumentation to users in the research community.
Patrick C.H. Lo, PhD
Senior Editor, BioTechniques