NovoCyte Quanteon™ Flow Cytometer
When Exceptional Performance Meets Simplicity.
When Exceptional Performance Meets Simplicity.
Flow Cytometry continues to evolve and advance as a must-have cell analysis tool, along with emerging breakthrough techniques such as CRISPR for gene editing, Immune checkpoint blockade for cancer treatment, etc.
Built upon the success of NovoCyte® flow cytometers (1~3 lasers, up to 15 fluorescent channels) since 2014, ACEA Biosciences now provides the next generation and most advanced benchtop flow cytometer NovoCyte QuanteonTM, a 4-laser flow cytometer with up to 25 fluorescent channels.
Utilized by all experience levels of Flow users, the 1~4 lasers, up to 25 colors intuitive, flexible and upgradable NovoCyte Flow Cytometry solution will continue to demystify FLOW and broaden its applications in curing cancer, understanding the microbiome, regenerative and precision medicines, and safeguarding human health, etc.
The NovoCyte Quanteon is a breakthrough in flow cytometry design in that it provides 25 fluorescent channel capability, with light collected against 25 different detectors. Since the detectors have a 7 log dynamic range, coupled with advanced auto compensation features, the need to tune each detector has been completely alleviated. You now have the flexibility to choose from 25 fluorescent channels utilizing four lasers, without having to sacrifice the performance in one channel, for another channel.
The fluorescent channels available on the NovoCyte Quanteon from ACEA include:
The proprietary optical detection system found inside the NovoCyte Quanteon employs a series of 25 Silicon photomultipliers (SiPM), which have superb detection sensitivity, at the photon level. The novel design provides highly discrete management of each fluorescent channel, superior optical detection, and cross-talk between channels is completely minimized. The NovoCyte Quanteon also has an automatic optical filter recognition feature, which allows users to efficiently change filter configurations, as needed by the user. The system has a fail-safe hardware feature, which conveniently alerts the user to any improperly arranged filter configurations, thereby mitigating against potential hardware errors during system set-up, and makes it ideal for a multi-user environment.
What is a SiPM?
Silicon photomultipliers (SiPM) are solid-state, silicon-substrate-based, photon-level-sensitive semiconductor devices, with a 7 log dynamic range. Consisting of a compact array of avalanche photodiodes operating in unison, the SiPM is a compact detector with photon counting capability. Innovative optics design in NovoCyte Quanteon incorporates 25 independent SiPM for collecting and processing signals for each of its fluorescence channels.
Equipped with high quality lasers, optical filters and detectors to ensure consistent signal detection, and combined with fluidic feedback control mechanisms to maintain steady flow rates, the NovoCyte Quanteon is the flow cytometer you can always rely on. It has demonstrated superior stability across a wide range of sample flow rates, a critical requirement for a high end flow cytometer to provide consistent results under variable operating conditions. The NovoCyte Quanteon gives you peace of mind so you can focus more on your experiments.
Direct Absolute Cell Count Makes Reference Beads Obsolete
NovoCyte Quanteon uses a high accuracy syringe pump to drive the sample, directly providing accurate absolute count results in every single run.
Superior Reproducibility & Stability of Quanteon’s Fluidic System
|The Quanteon’s fluidic system is designed to deliver superior performance. When compared to other flow cytometers, the fluidic consistency and stability of the Quanteon is unmatched. Other instruments utilizing peristaltic pumps are often plagued with fluidic pulsation, causing inconsistency and inaccuracy in absolute cell counts.|
The NovoCyte Quanteon provides enhanced automation capabilities with faster and higher throughput:
The new 1.3.0 version of NovoExpress® software supports the new NovoCyte Quanteon flow cytometers and NovoSampler® Q autosampler products, in addition to NovoCyte® flow cytometer and NovoSampler® Pro products.
It is an easy-to-use and highly intuitive software package for both Flow Cytometry data collection and analysis, it provides a powerful range of professional flow cytometry data analysis functionalities, as well as turn-key data analysis functions.
The highlighted features include:
Equipped with 25 fluorescence channels from 4 lasers (405, 488, 561, and 637nm), the Quanteon offers tremendous flexibility in panel design. Optimized detector settings eliminate the need for complicated and laborious adjustments, making data acquisition as simple as load-and-go.
Figure 7: Above, we show an 18-color pan-leukocyte immunophenotyping panel on PBMCs using the NovoCyte Quanteon, adapted from OMIP-024 (Cytometry Part A, 85A:995-998, 2014). This panel was designed to monitor different immune subsets with activation and differentiation markers.
NovoCyte Quanteon’s FSC/SSC detection optics and signal processing electronics have been optimized to resolve particles of size down to 0.1 μm. With such superior resolution, platelets, bacteria, and various sub-micron particles can be readily identified and analyzed.
Figure 8: NovoCyte Quanteon Flow Cytometer for 0.1~50 µm size particle detection
|2010097||ACEA NovoCyte Quanteon™ 4025 Flow Cytometer (4 Lasers, 25 FL channels)|
|2010099||ACEA NovoCyte Quanteon™ 4020 Flow Cytometer (4 Lasers, 20 FL channels)|
|2010100||ACEA NovoCyte Quanteon™ 4016 Flow Cytometer (4 Lasers, 16 FL channels)|
|2010101||ACEA NovoCyte Quanteon™ 4000C (4 Lasers, 16~25 FL channels) Custom Build Flow Cytometer|
|2010098||ACEA NovoCyte Quanteon™ 4025 Flow Cytometer (4 Lasers, 25 FL channels) and NovoSampler® Q Combo|
|2010102||ACEA NovoCyte Quanteon™ 4020 Flow Cytometer (4 Lasers, 20 FL channels) and NovoSampler® Q Combo|
|2010103||ACEA NovoCyte Quanteon™ 4016 Flow Cytometer (4 Lasers, 16 FL channels) and NovoSampler® Q Combo|
|2010104||ACEA NovoCyte Quanteon™ 4000C Custom Build Flow Cytometer and NovoSampler® Q Combo|
|2020098||ACEA NovoSampler® Q (For NovoCyte Quanteon only)|