NovoCyte Quanteon™ Flow Cytometer

When Exceptional Performance Meets Simplicity.

NovoCyte Quanteon 360 view

Flow Cytometry continues to evolve and advance as a must-have cell analysis tool, along with emerging breakthrough techniques such as CRISPR for gene editing, Immune checkpoint blockade for cancer treatment, etc.

Built upon the success of NovoCyte® flow cytometers (1~3 lasers, up to 15 fluorescent channels) since 2014, ACEA Biosciences now provides the next generation and most advanced benchtop flow cytometer NovoCyte QuanteonTM, a 4-laser flow cytometer with up to 25 fluorescent channels.

Utilized by all experience levels of Flow users, the 1~4 lasers, up to 25 colors intuitive, flexible and upgradable NovoCyte Flow Cytometry solution will continue to demystify FLOW and broaden its applications in curing cancer, understanding the microbiome, regenerative and precision medicines, and safeguarding human health, etc.

Take the “Quanteon” leap to reap the benefits exceeding your needs for simplified single cell, high throughput, multiparametric cell analysis


Exquisite sensitivity for dim signal and rare cell/particle population

  • 4 lasers, 16~25 colors’ flexible configurations allow customization for versatile applications, along with the 1~3 lasers, up to 15 colors NovoCyte systems
  • Most sensitive 25 independent silicon photomultiplier (SiPM) detectors for each of all 25 fluorescent channels – 1st ever innovative SiPM integration into Flow Cytometry
  • Unprecedented 100nm small particle detection resolution for extracellular vesicles, exosome, virus studies
“Amazing instrument, it is the future of flow cytometry!”
“I have only wonderful things to say about the Novocyte. It is incredibly easy to use, we get reproducible results, the software is the best one out there, the maintenance is super easy, and ACEA has excellent technical support! Can’t wait to upgrade to the 25-channel instrument!“Luz M. Londoño, Halozyme Therapeutics Inc. (review from SelectScience)


Transformative simplicity for all experience levels of Flow users

  • Integrated Flow Cytometry solution combines operational simplicity, automation with the most intuitive yet powerful NovoExpress software
  • Time-saving from simultaneous data collection and analysis for routing or high complexity experiments
  • Coupled with auto-compensation, 7.2-log wide dynamic range eliminates the need for routine detector adjustments
“Software is straightforward, and the software interface is easy to handle”
“This (NovoExpress) software is straightforward, and the software interface is easy to handle. The implemented auto compensation and hierarchic tree structure is a highlight for effective organization of experimental data.”Matthias Schiemann, Technische Universität München


Hassle-free convenience with the high-throughput and most reliable autosampler

  • Convenient compatibility with your sample format (40-tube rack, 24-, 48-, 96-, and 384-well plates) coupled with an easy switch between manual and automation modes
  • Blazing fast time-to-result: 96-well plate throughput <20 minutes
  • Exceptional operational accuracy & efficiency: <0.1% carryover, embedded barcode reader, LIS compatibility, etc.
“My best friend in the lab!!!”
“I use the NovoCyte to screen compounds for induction of micronuclei in the TK6 cell line as part of our gene tox strategy. With the NovoSampler and 96-well format, the assay is extremely robust and the results reproducible to a very high degree. The NovoCyte is user friendly and creating new applications to support our immune tox strategy has been delightfully easy, even for a novice within the field of immunology.”Joan Christiansen, H. Lundbeck A/S. (NovoCyte review from SelectScience)
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NovoCyte Quanteon Flow Cytometer with NovoSampler Q™ and fluidics station

Four Lasers, 25 Colors Plus Scatter Parameters

The NovoCyte Quanteon is a breakthrough in flow cytometry design in that it provides 25 fluorescent channel capability, with light collected against 25 different detectors. Since the detectors have a 7 log dynamic range, coupled with advanced auto compensation features, the need to tune each detector has been completely alleviated. You now have the flexibility to choose from 25 fluorescent channels utilizing four lasers, without having to sacrifice the performance in one channel, for another channel.

The fluorescent channels available on the NovoCyte Quanteon from ACEA include:

NovoCyte Quanteon fluorescent channels

Advanced Optical Detection and Flexible Configuration

The proprietary optical detection system found inside the NovoCyte Quanteon employs a series of 25 Silicon photomultipliers (SiPM), which have superb detection sensitivity, at the photon level. The novel design provides highly discrete management of each fluorescent channel, superior optical detection, and cross-talk between channels is completely minimized. The NovoCyte Quanteon also has an automatic optical filter recognition feature, which allows users to efficiently change filter configurations, as needed by the user. The system has a fail-safe hardware feature, which conveniently alerts the user to any improperly arranged filter configurations, thereby mitigating against potential hardware errors during system set-up, and makes it ideal for a multi-user environment.

Silicon Photo Multiplier (SiPM) – The Most Sensitive Photodetector in the Market is Now in the Quanteon

What is a SiPM?

Silicon Photo Multiplier (SiPM)

Silicon photomultipliers (SiPM) are solid-state, silicon-substrate-based, photon-level-sensitive semiconductor devices, with a 7 log dynamic range. Consisting of a compact array of avalanche photodiodes operating in unison, the SiPM is a compact detector with photon counting capability. Innovative optics design in NovoCyte Quanteon incorporates 25 independent SiPM for collecting and processing signals for each of its fluorescence channels.

SiPM’s strengths:

  • Superior photon detection sensitivity
  • High gain and high quantum efficiency
  • Instantaneous warm up and fast response
  • Robust and long lifespan
  • High durability

flow cytometry software with 7 log dynamic range

Exceptional Reliability Provides Consistent Results, Fast or Slow

Equipped with high quality lasers, optical filters and detectors to ensure consistent signal detection, and combined with fluidic feedback control mechanisms to maintain steady flow rates, the NovoCyte Quanteon is the flow cytometer you can always rely on. It has demonstrated superior stability across a wide range of sample flow rates, a critical requirement for a high end flow cytometer to provide consistent results under variable operating conditions. The NovoCyte Quanteon gives you peace of mind so you can focus more on your experiments.

Direct Absolute Cell Count Makes Reference Beads Obsolete

NovoCyte Quanteon uses a high accuracy syringe pump to drive the sample, directly providing accurate absolute count results in every single run.

  • Volumetric absolute count is determined for each and every sample automatically
  • Complicated calibration of the fluidics system is not required
  • Expensive reference beads are not required



Superior Reproducibility & Stability of Quanteon’s Fluidic System

The Quanteon’s fluidic system is designed to deliver superior performance. When compared to other flow cytometers, the fluidic consistency and stability of the Quanteon is unmatched. Other instruments utilizing peristaltic pumps are often plagued with fluidic pulsation, causing inconsistency and inaccuracy in absolute cell counts.

Walk-away Automations Capabilities

The NovoCyte Quanteon provides enhanced automation capabilities with faster and higher throughput:

  • Quick startup and pre-scheduled shutdown
  • Hassle-free fluidics allows for automatic clog detection and recovery
  • “Smart” optical filters are automatically recognized by the instrument to ensure correct configuration with easy filter swapping
  • Fluidic station capable of signaling low fluid or high waste levels to eliminate the need of manual inspection. Fluidics consumption is estimated before plate runs to ensure uninterrupted sample acquisition
  • Integrated bar code scanner to read plate information
  • Support various sample formats (40 tube rack, 24/48/96/384 well plates) including customizable plates
  • High performance orbital shaker supports the programmable setup of maintaining cell suspension during sample multi-sample acquisition


Powerful Software Modules

The new 1.3.0 version of NovoExpress® software supports the new NovoCyte Quanteon flow cytometers and  NovoSampler® Q autosampler products, in addition to NovoCyte® flow cytometer and NovoSampler® Pro products.

It is an easy-to-use and highly intuitive software package for both Flow Cytometry data collection and analysis, it provides a powerful range of professional flow cytometry data analysis functionalities, as well as turn-key data analysis functions.

The highlighted features include:

  • One software interface for all: combining sample acquisition and data analysis
  • Analyze acquired data in real time during flow experiment to maximize productivity and efficiency
  • Customizable statistical parameters with live updates when running samples
  • Powerful compensation tools and convenient adjustments allow accurate pre- and post-acquisition compensation
  • Batch analysis and reporting
  • Easily create publication-quality figures with customizable plot scale, font, and legend
  • Export as FCS (3.0, 3.1) or CSV files, import FCS files for analysis

  • Cell Cycle Analysis Module
    • Dean Jett Fox (DJF) and Watson Pragmatic algorithms are both incorporated in the NovoExpress cell cycle analysis module. This provides additional flexibility for your cell cycle analysis and quantitation of G1, S, and G2/M transitions, as well as other parameters such as CV’s and G2/G1 ratio.
  • Cell Proliferation Modeling
    • Cell proliferation is studied to understand compound toxicity, drug effects on tumor cell growth, immune cell behavior under different conditions, etc. Flow cytometry is ideal for measuring cell proliferation and studying unique cell types within a heterogeneous population providing more information than can be obtained in other commonly used assays.
      Automatic analysis of cell proliferation to quickly identify generations of cell division and calculate the proliferation index for easy quantitation.
  • Heat map data representation
    • A heat map is a good representation of data statistics in the form of a map in which data values are represented as colors. Color representation of user-defined parameters allow quick visualization and comparison of many samples simultaneously.

Up to 27 Parameter Analysis

Equipped with 25 fluorescence channels from 4 lasers (405, 488, 561, and 637nm), the Quanteon offers tremendous flexibility in panel design. Optimized detector settings eliminate the need for complicated and laborious adjustments, making data acquisition as simple as load-and-go.

Figure 7: Above, we show an 18-color pan-leukocyte immunophenotyping panel on PBMCs using the NovoCyte Quanteon, adapted from OMIP-024 (Cytometry Part A, 85A:995-998, 2014). This panel was designed to monitor different immune subsets with activation and differentiation markers.

100 nm Small Particle Resolution

NovoCyte Quanteon’s FSC/SSC detection optics and signal processing electronics have been optimized to resolve particles of size down to 0.1 μm. With such superior resolution, platelets, bacteria, and various sub-micron particles can be readily identified and analyzed.

NovoCyte Quanteon Flow Cytometer for 0.1~50 µm size particle detection

Figure 8: NovoCyte Quanteon Flow Cytometer for 0.1~50 µm size particle detection

Ordering Info

Cat. No.Description
2010097ACEA NovoCyte Quanteon™ 4025 Flow Cytometer  (4 Lasers, 25 FL channels)
2010099ACEA NovoCyte Quanteon™ 4020 Flow Cytometer (4 Lasers, 20 FL channels)
2010100ACEA NovoCyte Quanteon™ 4016 Flow Cytometer (4 Lasers, 16 FL channels)
2010101ACEA NovoCyte Quanteon™ 4000C (4 Lasers, 16~25 FL channels) Custom Build Flow Cytometer 
2010098ACEA NovoCyte Quanteon™ 4025 Flow Cytometer (4 Lasers, 25 FL channels) and NovoSampler® Q Combo
2010102ACEA NovoCyte Quanteon™ 4020 Flow Cytometer (4 Lasers, 20 FL channels) and NovoSampler® Q Combo
2010103ACEA NovoCyte Quanteon™ 4016 Flow Cytometer (4 Lasers, 16 FL channels) and NovoSampler® Q Combo
2010104ACEA NovoCyte Quanteon™ 4000C Custom Build Flow Cytometer and NovoSampler® Q Combo
2020098ACEA NovoSampler® Q (For NovoCyte Quanteon only)