Products NovoCyte Flow Cytometer
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NovoCyte Flow Cytometer

Incredibly flexible high performer. Plenty of automation options. The NovoCyte benchtop flow cytometer supports a variety of multi-parametric experiments, at a price point labs welcome.

NovoCyte® is a high performance benchtop flow cytometer designed for all levels of users and all types of laboratories. This budget-friendly instrument is capable of detecting up to 17 parameters with enhanced sensitivity and resolution. The customizable laser and optical configurations of NovoCyte offer a high degree of flexibility while providing complex cell analysis capabilities. The NovoExpress® software facilitates easy and intuitive sample acquisition and analysis. Automation of multiple fluidic functions eliminates cumbersome and time consuming procedures. User hands-on time is minimized by the flexible NovoSampler® Pro which can automatically analyze samples in single tubes, multi-tube racks, or 24-, 48-, or 96-well plates.


Address the full range of current and future multi-parameter analysis research needs with the NovoCyte flow cytometer. ACEA brings researchers high performance flow cytometry at a low investment cost with the NovoCyte platform.

ACEA offers a system which is:

  • Powerful- up to 17 parameter detection with enhanced sensitivity and resolution.
  • Intuitive – automated instrument maintenance functions and advanced data analysis capabilities for greater usability.
  • Customizable – 1 to 3 laser options, exchangeable filters, multiple sampling options, and flexible analysis formats.

Other important features enhance usability, such as fixed optical alignment, 24-bit detection dynamic ranges with no need for PMT voltage adjustment, accurate volumetric-based cell counting, pressure sensors to monitor fluidic status in real-time, and automatic cleaning and decontamination processes.

ACEA also developed the NovoExpress software, which is intuitive and easy to use. Flexible analysis templates and plotting tools offer enhanced data analysis efficiency.

NEW NovoCyte Configuration          Click Here

Click here to download our Multi-option Configuration Guide.

Configurable Laser Systems

NovoCyte Violet/Blue/Red  Laser Default Systems

NovoCyte Violet/Yellow/Blue and Red/Yellow/Blue Laser Default Systems

NovoCyte Technical Specifications

Specification and performance claims would be based on certain specific experiment conditions.

Optics

Laser Configuration Spatially separated beams with 10 x 80 μm elliptical spots
Optical Alignment Procedure Fixed, no operator alignment required
Flow Cell 170 x 290 μm rectangular quartz flow cell
Scatter Resolution 0.2 μm
Specification and performance claims would be based on certain specific experiment conditions.
Cell Size 0.2 – 50 μm
Fluorescence Threshold Sensitivity FITC < 75 MESF; PE < 50 MESF; APC <20 MESF
Fluorescence Resolution < 3% CV for CEN
Filters User Exchangeable

Fluidics

Sample Acquisition Rate 35,000 events/second
Volumetric Absolute Count Precision Syringe Pump: CV < 5%
Sample Flow Rate 5-120 μL/min
Sheath Flow Rate 6.5 mL/min
Sample Aspiration Volume 10 μL – 5 mL
Fluid Container Capacity 3 L sheath, 3 L waste, 500 mL cleaning, 500 mL decontamination
Carryover < 0.1%
Fluidics Maintenance Automated startup, cleaning, decontamination and shutdown

Data Processing

Parameters Height and Area for FSC, SSC and all Fluorescence Channels, Width and Time
Dynamic Range 24 bit; 7.2 decades logarithmic scale; no need for PMT voltage adjustment
Compensation Automatic compensation, manual compensation, visual compensation tools available for pre/post/live acquisitions
Output Data Files FCS 3.1, NovoExpress (.ncf), PDF reports, bitmap graphics, vector graphics, CSV
Workstation Dell OptiPlex 7040 SFF or other compatible models, 1 TB with 23.8″ LCD monitor
Computer Operating System Microsoft Windows® 7 Professional (64 bit), Microsoft Office® 2016
Software ACEA NovoExpress®

Sampling

Manual Sample Loading 12 X 75 mm tube, 1.5 mL Eppendorf tube
Automatic Sample Loading Optional – compatible with 12 x 75 mm tube, 1.5 & 2 mL tubes, “bullet” tubes in 96-pos. racks, 24-well, 48-well and 96-well microtiter plates

Operating Conditions

Instrument Dimension (W X D X H) 23.6 x 17.7 x 15.4 in (60 x 45 x 39 cm)
Instrument Weight 86 lb (39 kg)
Power Requirements Input: 12 VDC, Power: 90 VA
Environment Requirements Temperature: 15-32˚C ; Relative Humidity: 80% maximum

Optimized PMT Voltage & 7 Decades Detection Dynamic Range

Provides 107 dynamic range for signal detection and processing, offering a broader signal range than other flow cytometers. Wide range of fluorescent and scattering signal intensities eliminates the need for complicated and laborious PMT voltage adjustment. Data acquisition is just load-and-go.

Configurable Fluorescence Detection Channels for Enhanced Assay Flexibility

Customizable selection and upgrade with lasers of different wavelengths allow for personalized, versatile choices of fluorochromes for flow cytometry assays. User interchangeable filters and dichroic mirrors broaden available fluorescence channels to expand users’ detection options.

15 Fluorescent Channel Detection: Multiple Laser Options Providing Flexibility of Choice (405nm, 488nm, 561nm, 640nm)

Customize the instrument to meet detection needs with blue, red and violet laser options. System can be upgraded as analytical requirements increase – no need to purchase another instrument. State-of-the-art solid-state lasers provide high quality and extremely stable optical illumination.

Novel Optical Design & Enhanced Signal Detection

Patent pending independent beam shaping optics and light collection system maximizes signal detection efficiency, increasing sensitivity and resolution for each fluorescence channel. Fixed optical alignment removes need for daily maintenance and adjustment of optical system, providing convenient operation and superior long-term stability. Optimized forward scatter obscuration bar increases sensitivity. Enhanced small particle detection and resolution as a result of optimized obscuration bar design.

Volumetric Fluidics System for Increased Accuracy & Easy Maintenance

The NovoCyte flow cytometer uses a highly accurate, volumetric syringe pump which enables direct absolute counting of samples, eliminating the need to use expensive reference beads.
volumetric-count
Real-time monitoring of fluidics prevents flow path obstruction. Accurate pressure sensors monitor fluidic status in real-time, thus the possibility of flow path obstruction is greatly reduced, allowing for reliable and accurate data acquisition. Automated SIP (sample injection probe) washing following sample acquisition minimizes sample carryover and increases capability to detect rare events. High speed data acquisition of 35,000 events per second.
QC
Routine maintenance features are as simple as clicking a button and following the on screen instructions

High Sensitivity & High Resolution Detection

Highly efficient light collection ensures reliable detection of sub-micron particles and dim signals.

Using innovative optical design for light collection and high quality PMTs, fluorescence signals are efficiently detected with exceptionally high signal-to-noise ratio, ensuring a high detection sensitivity for weak positive samples and small particles.
img_feature2

High Fluorescent Signal Resolution Improves Detection Accuracy

Advanced optical and fluidic system design, premier quality components, and innovative signal processing algorithms collectively ensure accurate extraction of detection parameters with consistently low variation.
Spherotech8

Option for High-Throughput and Automated Analysis of Multiple Samples

The NovoSampler is a modular autosampler, that can be interchanged with the single tube loader in just a couple minutes, is compatible with 12 x 75mm tube, 1.5 & 2mL tubes and 24/48/96 well plates, allowing for versatile loading modes, and increased throughput. Automatic calibration is just the push of a button and this procedure ensures accuracy between different plate types. Simply select your plate, identify your wells or tubes to run, and start running. The NovoSampler will automatically switch between samples, employ mixing or rinsing steps between samples if desired, and complete your run all while you are away.


Experiment Analysis Design

Experiment Manager provides a schematic view of the sample analysis being performed.This allows for access to cytometer setting,compensation matrix, report generation, and data analysis. Multiple samples can be analyzed with the same settings by a simple drag-and-drop template function.

Automated & Versatile Data Analysis Functions

Powerful NovoExpress software allows efficient data acquisition, data analysis and report generation. NovoExpress software provides flexible analysis templates and plotting tools, offering enhanced data analysis efficiency.

NovoExpress Download

Download NovoExpress Software Now!

DataAnalysis

Pre & Post Acquisition Compensation

Instantaneous compensation results can be simply achieved by adjustment of the compensation scaling bar on the plot, allowing rapid and accurate compensation, thus eliminating tedious trial and error adjustments of compensation matrix coefficients.
A simple click of a button initiates the automated cleaning and de-contamination process and effectively removes any residual samples in the fluidics path, saving users’ valuable time from laborious manual cleaning and decontamination routines.

QC2

Rapid Import & Export of Data Files


Applications Overview

NovoCyte’s expandable laser capabilities along with configurable optical detection design guarantee scientists the ability to advance research and to explore new applications in various areas of cellular analysis. NovoCyte provides a user friendly software interface enabling fast data acquisition and analysis of various flow cytometric applications.

Apoptosis

Apoptosis, or programmed cell death, is the process by which cells regulate how they die, activating specific pathways that cause the cell to shrink, condense, and eventually be cleared by phagocytosis. This is in contrast to necrotic cell death where cells die uncontrollably and fall apart, which can lead to detrimental effects such as the activation of an immune response. Therefore, apoptotic cells that die in a very orderly fashion limit disruption of nearby cells and tissue.
There are many ways to measure cell death and distinguish it from apoptosis or necrosis. These assays are easily quantified using the NovoCyte flow cytometer due to automatic compensation settings and a wide dynamic range of fluorescence detection which eliminates the need for any PMT voltage adjustments

Cell Cycle Analysis

Normal human somatic cells are diploids containing a constant amount of DNA. During cell cycle progression, DNA synthesis results in a doubling of total DNA content, followed by restoration of the normal DNA content after mitosis. With the NovoCyte flow cytometer, detailed cell cycle analysis can be performed to understand tumor cell differentiation, cell transformation and cell-compound interaction.

 

Cell Cycle Analysis Figure

Cell Signaling

Flow cytometry has traditionally been used to detect extracellular proteins to identify different cell populations. With the advancement of technology and reagents in recent years, we now are able to easily detect intracellular proteins in individual cells. One very useful application for the detection of intracellular proteins is to measure specific cells signaling pathways. These pathways are often altered in disease states, such as cancer, and are now easily detected using flow cytometry providing single cell analysis of these signaling changes.
percp

Jurkat cells were either left in culture(pERK unstim) or stimulated with cell stimulation cocktail for 1hr (pERK +stim).  Cells were washed, fixed and permeablized, followed by staining with anti-phospo-ERK1/2 (T204/Y202) PerCP antibody for 1hr.  Cells were washed and analyzed on the NovoCyte flow cytometer for phospho-ERK levels and compared using a histogram overlay generated with the NovoExpress acquisition and analysis software.

Immunophenotyping

Immunophenotyping quickly identifies candidate cell types, sub-classes and functions. The ACEA NovoCyte flow cytometer can be used for multi-parameter lymphocyte sub-population counting and phenotyping, helper T lymphocyte differentiation and functional study, new sub-population identification, disease specific sub-population identification, and cell surface marker detection.

immunophenotyping

Intracellular Protein Detection

Flow cytometry has traditionally been used to detect extracellular proteins to identify different cell populations. With the advancement of technology and reagents in recent years, we now are able to easily detect intracellular proteins in individual cells. One very useful application for the detection of intracellular proteins is to measure specific cells signaling pathways. These pathways are often altered in disease states, such as cancer, and are now easily detected using flow cytometry providing single cell analysis of these signaling changes.
percp

Jurkat cells were either left in culture(pERK unstim) or stimulated with cell stimulation cocktail for 1hr (pERK +stim).  Cells were washed, fixed and permeablized, followed by staining with anti-phospo-ERK1/2 (T204/Y202) PerCP antibody for 1hr.  Cells were washed and analyzed on the NovoCyte flow cytometer for phospho-ERK levels and compared using a histogram overlay generated with the NovoExpress acquisition and analysis software.

Proliferation

Cell proliferation is an essential function and highly structured event that when unregulated, can cause disease. We can measure proliferation through absolute cell counts or with a dye, such as CFSE. When cells labeled with CFSE divide, the dye is partitioned equally between daughter cells and we can measure the loss of CFSE fluorescence over time as the dye is continuously diluted. The mean fluorescence intensity (MFI) of the dye was also plotted with cell concentration over time to show the inverse relationship between the two. This type of assay is often used to look at changes in T lymphocyte activation.

 

App Note 6 Image

Application Notes

Brochures

Spec Sheets

NovoCyte QC Particles Lot File

Introduction:

1. Download the QC particles lot file corresponding to the lot number of the QC particles.
2. Save the QC particles lot file to the “\QC\QC Beads” folder in the NovoExpress installation directory ( the default installation directory for NovoExpressTM is C:\Program Files (x86)\NovoExpress ).
3. Run NovoCyte QC test.

Downloads:

Note: Please upgrade NovoExpress to 1.0.2 or above version to use the QC particles lot files.(Right click on the corresponding lot number, choose " save link as ", and save to local)

Videos

Live Technical Webinar: Measuring Calcium Flux by Flow CytometryAccurate measurements of intracellular Ca2+ concentrations allows for a more comprehensive understanding of Ca2+ regulated cell functions and signaling pathways. Ca2+ is an important molecule that affects a wide range of physiological and pathological processes. Unlike other secondary messengers, Ca2+ is not synthesized or metabolized, but stored and rapidly released by channels/pumps that maintain Ca2+ concentrations in distinct cellular compartments. ... Read More

Accurate measurements of intracellular Ca2+ concentrations allows for a more comprehensive understanding of Ca2+ regulated cell functions and signaling pathways. Ca2+ is an important molecule that affects a wide range of physiological and pathological processes. Unlike other secondary messengers, Ca2+ is not synthesized or metabolized, but stored and rapidly released by channels/pumps that maintain Ca2+ concentrations in distinct cellular compartments. Flow cytometry allows real time measurement of a calcium flux response utilizing fluorescent Ca2+ indicators which exhibit an increase in fluorescence upon binding to Ca2+. In this webinar we will discuss the basics of calcium flux measurements by flow cytometry, providing specific details on experiment setup, acquisition, and analysis on the NovoCyte flow cytometer.

Key topics to be covered:
1. Essentials of calcium measurements by flow cytometer
2. Calcium flux experimental setup and acquisition on the NovoCyte
3. Detailed analysis of calcium flux in a T cell line using calcium indicator dye Fluo-4/AM

Learn More about the NovoCyte Flow Cytometer       Click here

Speaker:

Lauren Jachimowicz, Ph.D. 
Application Development Scientist, ACEA Biosciences, Inc.
Lauren Jachimowicz received her PhD in Biological Science from the University of California, San Diego where she studied the role of the circadian clock genes in T cells. Currently, she is the Application Development Scientist for flow cytometry at ACEA Biosciences advancing flow cytometry into new fields of research.

 

 

WEBINAR: Flow Cytometric Assessment, Quantification and Regulation of Human Neutrophil Extracellular Traps (NETS)At the CYTO 2017 meeting Dr. Adriano Rossi and Dr. Calum Robb from the University of Edinburgh described the development of a new flow cytometry-based assay for studying NETosis, a cell death process that occurs when stimulated neutrophils cast an extracellular web containing DNA, histones, and anti-bacterial proteins that ensnare/neutralize invading organisms. Using the NovoCyte® flow cytometer, they demonstrate the ... Read More


At the CYTO 2017 meeting Dr. Adriano Rossi and Dr. Calum Robb from the University of Edinburgh described the development of a new flow cytometry-based assay for studying NETosis, a cell death process that occurs when stimulated neutrophils cast an extracellular web containing DNA, histones, and anti-bacterial proteins that ensnare/neutralize invading organisms. Using the NovoCyte® flow cytometer, they demonstrate the detection and quantification of neutrophil extracellular traps (NETs), as well as analysis of the regulatory mechanisms involved.

WEBINAR: Flow Cytometry: Simplifying Assay Panel Design and WorkFLOWAbout this Webinar Researchers and flow cytometry users spend a significant amount of time designing flow assay panels and analyzing their samples. In this webinar, viewers will have the chance to learn about  tools and recent developments in flow cytometry  that simplify assay design and workflow. Our speakers will explore the capabilities of benchtop and cost-effective flow cytometry systems and ... Read More

About this Webinar

Researchers and flow cytometry users spend a significant amount of time designing flow assay panels and analyzing their samples.

In this webinar, viewers will have the chance to learn about  tools and recent developments in flow cytometry  that simplify assay design and workflow. Our speakers will explore the capabilities of benchtop and cost-effective flow cytometry systems and the easiest way to design flow panels.

Webinar Features

  • Design and capabilities of a NovoCyte flow cytometer
  • New features that simplify setup, acquisition, and analysis
  • How to design multicolor fluorescent panels using FluoroFinder’s experiment design platform
  • Tips for building better panels in less time

Featured Speakers:

Garret Guenther, Ph.D.
ACEA Biosciences.

Jeff Jensen, Ph.D.
FluoroFinder, LLC

 

NovoCyte Webinar Series – Measuring Apoptosis by Flow CytometryApoptosis, or programmed cell death, is a tightly regulated cellular phenomenon which includes many different signaling and effector pathways. By interrogating these processes in individual cells, and by evaluating large numbers of cells rapidly, flow cytometry can efficiently Read More

WEBINAR – Flow Cytometry: Emerging Instrumentation and Application Trends | Wed, Feb 15, 2017Flow cytometry remains an essential tool for cell analysis. Although often relegated to specialized core facilities, flow cytometry instruments are in fact becoming increasingly commonplace in life science labs. In this special webinar, viewers will have the chance to learn about recent developments in flow cytometry technology, including the emergence of lower cost, smaller flow cytometry options with the capability of analyzing multiple fluorescent colors. Our speakers will explore ... Read More

Flow cytometry remains an essential tool for cell analysis. Although often relegated to specialized core facilities, flow cytometry instruments are in fact becoming increasingly commonplace in life science labs.

In this special webinar, viewers will have the chance to learn about recent developments in flow cytometry technology, including the emergence of lower cost, smaller flow cytometry options with the capability of analyzing multiple fluorescent colors. Our speakers will explore the design, development, and uses for smaller, more cost-effective flow cytometry systems in research labs. In this webinar, you will gain insights on:

  • Design and development of the latest multicolor flow cytometry instruments
  • Application of flow cytometry techniques for different experimental needs
  • How analyzing multiple cellular parameters using different  fluorescent colors can extend experimental possibilities
  • How to effectively analyze and interpret your flow cytometry data sets

Learn More about the NovoCyte Flow Cytometer       Click here

Speakers:

Paul K. Wallace, PhD
Roswell Park Cancer Institute
Paul Wallace has served since 2003 as Director of the Flow and Image Cytometry Department at the Roswell Park Cancer Institute (RPCI) in Buffalo, NY, where he is a Professor of Oncology. He is also an Associate Professor of Biotechnical and Clinical Laboratory Sciences at the University at Buffalo. His primary expertise and board certification is in the flow cytometric diagnosis of hematological malignancies.

Christopher Groves 
MedImmune, Inc. 
Christopher Groves is a Senior Manager at MedImmune Inc., where he heads a keystone technology group  challenged with expanding knowledge of disease mechanisms while also delivering innovative solutions in drug discovery and manufacturing that provide scientists access to cutting-edge technological capability.

William Telford, PhD 
Experimental Transplantation and Immunology Branch, NCI-NIH 
William Telford is the Head of the NCI-NIH Flow Cytometry core facility, which provides state-of-the-art flow cytometry as well as imaging technology and instrumentation to users in the research community.

Moderator:

Patrick C.H. Lo, PhD 
Senior Editor, BioTechniques

Videos: Product Overviews

Videos: Research Presentations

Live Technical Webinar: Measuring Calcium Flux by Flow CytometryAccurate measurements of intracellular Ca2+ concentrations allows for a more comprehensive understanding of Ca2+ regulated cell functions and signaling pathways. Ca2+ is an important molecule that affects a wide range of physiological and pathological processes. Unlike other secondary messengers, Ca2+ is not synthesized or metabolized, but stored and rapidly released by channels/pumps that maintain Ca2+ concentrations in distinct cellular compartments. ... Read More

Accurate measurements of intracellular Ca2+ concentrations allows for a more comprehensive understanding of Ca2+ regulated cell functions and signaling pathways. Ca2+ is an important molecule that affects a wide range of physiological and pathological processes. Unlike other secondary messengers, Ca2+ is not synthesized or metabolized, but stored and rapidly released by channels/pumps that maintain Ca2+ concentrations in distinct cellular compartments. Flow cytometry allows real time measurement of a calcium flux response utilizing fluorescent Ca2+ indicators which exhibit an increase in fluorescence upon binding to Ca2+. In this webinar we will discuss the basics of calcium flux measurements by flow cytometry, providing specific details on experiment setup, acquisition, and analysis on the NovoCyte flow cytometer.

Key topics to be covered:
1. Essentials of calcium measurements by flow cytometer
2. Calcium flux experimental setup and acquisition on the NovoCyte
3. Detailed analysis of calcium flux in a T cell line using calcium indicator dye Fluo-4/AM

Learn More about the NovoCyte Flow Cytometer       Click here

Speaker:

Lauren Jachimowicz, Ph.D. 
Application Development Scientist, ACEA Biosciences, Inc.
Lauren Jachimowicz received her PhD in Biological Science from the University of California, San Diego where she studied the role of the circadian clock genes in T cells. Currently, she is the Application Development Scientist for flow cytometry at ACEA Biosciences advancing flow cytometry into new fields of research.

 

 

WEBINAR: Flow Cytometric Assessment, Quantification and Regulation of Human Neutrophil Extracellular Traps (NETS)At the CYTO 2017 meeting Dr. Adriano Rossi and Dr. Calum Robb from the University of Edinburgh described the development of a new flow cytometry-based assay for studying NETosis, a cell death process that occurs when stimulated neutrophils cast an extracellular web containing DNA, histones, and anti-bacterial proteins that ensnare/neutralize invading organisms. Using the NovoCyte® flow cytometer, they demonstrate the ... Read More


At the CYTO 2017 meeting Dr. Adriano Rossi and Dr. Calum Robb from the University of Edinburgh described the development of a new flow cytometry-based assay for studying NETosis, a cell death process that occurs when stimulated neutrophils cast an extracellular web containing DNA, histones, and anti-bacterial proteins that ensnare/neutralize invading organisms. Using the NovoCyte® flow cytometer, they demonstrate the detection and quantification of neutrophil extracellular traps (NETs), as well as analysis of the regulatory mechanisms involved.

WEBINAR: Flow Cytometry: Simplifying Assay Panel Design and WorkFLOWAbout this Webinar Researchers and flow cytometry users spend a significant amount of time designing flow assay panels and analyzing their samples. In this webinar, viewers will have the chance to learn about  tools and recent developments in flow cytometry  that simplify assay design and workflow. Our speakers will explore the capabilities of benchtop and cost-effective flow cytometry systems and ... Read More

About this Webinar

Researchers and flow cytometry users spend a significant amount of time designing flow assay panels and analyzing their samples.

In this webinar, viewers will have the chance to learn about  tools and recent developments in flow cytometry  that simplify assay design and workflow. Our speakers will explore the capabilities of benchtop and cost-effective flow cytometry systems and the easiest way to design flow panels.

Webinar Features

  • Design and capabilities of a NovoCyte flow cytometer
  • New features that simplify setup, acquisition, and analysis
  • How to design multicolor fluorescent panels using FluoroFinder’s experiment design platform
  • Tips for building better panels in less time

Featured Speakers:

Garret Guenther, Ph.D.
ACEA Biosciences.

Jeff Jensen, Ph.D.
FluoroFinder, LLC

 

NovoCyte Webinar Series – Measuring Apoptosis by Flow CytometryApoptosis, or programmed cell death, is a tightly regulated cellular phenomenon which includes many different signaling and effector pathways. By interrogating these processes in individual cells, and by evaluating large numbers of cells rapidly, flow cytometry can efficiently Read More

WEBINAR – Flow Cytometry: Emerging Instrumentation and Application Trends | Wed, Feb 15, 2017Flow cytometry remains an essential tool for cell analysis. Although often relegated to specialized core facilities, flow cytometry instruments are in fact becoming increasingly commonplace in life science labs. In this special webinar, viewers will have the chance to learn about recent developments in flow cytometry technology, including the emergence of lower cost, smaller flow cytometry options with the capability of analyzing multiple fluorescent colors. Our speakers will explore ... Read More

Flow cytometry remains an essential tool for cell analysis. Although often relegated to specialized core facilities, flow cytometry instruments are in fact becoming increasingly commonplace in life science labs.

In this special webinar, viewers will have the chance to learn about recent developments in flow cytometry technology, including the emergence of lower cost, smaller flow cytometry options with the capability of analyzing multiple fluorescent colors. Our speakers will explore the design, development, and uses for smaller, more cost-effective flow cytometry systems in research labs. In this webinar, you will gain insights on:

  • Design and development of the latest multicolor flow cytometry instruments
  • Application of flow cytometry techniques for different experimental needs
  • How analyzing multiple cellular parameters using different  fluorescent colors can extend experimental possibilities
  • How to effectively analyze and interpret your flow cytometry data sets

Learn More about the NovoCyte Flow Cytometer       Click here

Speakers:

Paul K. Wallace, PhD
Roswell Park Cancer Institute
Paul Wallace has served since 2003 as Director of the Flow and Image Cytometry Department at the Roswell Park Cancer Institute (RPCI) in Buffalo, NY, where he is a Professor of Oncology. He is also an Associate Professor of Biotechnical and Clinical Laboratory Sciences at the University at Buffalo. His primary expertise and board certification is in the flow cytometric diagnosis of hematological malignancies.

Christopher Groves 
MedImmune, Inc. 
Christopher Groves is a Senior Manager at MedImmune Inc., where he heads a keystone technology group  challenged with expanding knowledge of disease mechanisms while also delivering innovative solutions in drug discovery and manufacturing that provide scientists access to cutting-edge technological capability.

William Telford, PhD 
Experimental Transplantation and Immunology Branch, NCI-NIH 
William Telford is the Head of the NCI-NIH Flow Cytometry core facility, which provides state-of-the-art flow cytometry as well as imaging technology and instrumentation to users in the research community.

Moderator:

Patrick C.H. Lo, PhD 
Senior Editor, BioTechniques

Instrument – Includes Workstation & NovoExpress Software

Model Laser Configuration Description Cat. No.
ACEA NovoCyte® 1000 Blue Laser (488 nm) 3 color, FSC, SSC 2010001
ACEA NovoCyte® 2000R Blue / Red Laser (488/640 nm) 4 color, FSC, SSC 2010002
ACEA NovoCyte® 2060R Blue / Red Laser (488/640 nm) 6-7 color, FSC, SSC 2010004
ACEA NovoCyte® 3000 Violet/Blue/Red Laser (488/640/405 nm) 13 color, FSC, SSC 2010011
ACEA NovoCyte® 3000RYB Red / Yellow / Blue Laser (640/561/488 nm) 13-14 color, FSC, SSC 2010041
ACEA NovoCyte® 3000VYB Violet / Yellow / Blue Laser (405/561/488 nm) 14 color, FSC, SSC 2010042
ACEA NovoCyte® 2100YB Yellow / Blue Laser (561/488 nm) 10 color, FSC, SSC 2010043
ACEA NovoCyte® 1000 and NovoSampler® Pro Combo Blue Laser (488 nm) 3 color, FSC, SSC 2010047
ACEA NovoCyte® 2000 and NovoSampler® Pro Combo Blue / Red Laser (488/640 nm) 4 color, FSC, SSC 2010048
ACEA NovoCyte® 2060 and NovoSampler® Pro Combo Blue / Red Laser (488/640 nm) 6 color, FSC, SSC 2010049
ACEA NovoCyte® 3000 and NovoSampler® Pro Combo Blue / Red / Violet Laser (488/640/405 nm) 13 color, FSC, SSC 2010050
ACEA NovoCyte® 3000RYB and NovoSampler® Pro Combo Red / Yellow / Blue Laser (640/561/488 nm) 13 color, FSC, SSC 2010044
ACEA NovoCyte® 3000VYB and NovoSampler® Pro Combo Violet / Yellow / Blue Laser (405/561/488 nm) 13 color, FSC, SSC 2010045
ACEA NovoCyte® 2100YB and NovoSampler® Pro Combo Yellow / Blue Laser (561/488 nm) 10 color, FSC, SSC 2010046
ACEA NovoCyte® 2070V 7 FL Channels Blue / Violet Laser (445/530/572/695 nm) 7 color, FSC, SSC 2010025
ACEA NovoCyte® 2110V 11 FL Channels Blue / Violet Laser (445/530/572/660/695/780 nm) 11 color, FSC, SSC 2010027
ACEA NovoCyte® 1050 5 FL Channels Blue Laser (530/572/615/675/780 nm) 5 color, FSC, SSC 2010035
ACEA NovoCyte® 1040 4FL Channels Blue Laser (530/572/675/780 nm) 4 color, FSC, SSC 2010034
ACEA NovoCyte® 2070R Blue/Red Laser (488/640 nm) 7-8 Color, FSC, SSC 2010065
ACEA NovoCyte® 2090V Blue/Violet Laser (488/405 nm) 9 Color, FSC, SSC 2010067
ACEA NovoCyte® 3005 Violet/Blue/Red Laser (405/488/640 nm) 14-15 Color, FSC, SSC 2010064

Accessories, Reagents and Kits

Description Cat. No.
ACEA NovoSampler® Pro 2020070
NovoCyte® SIP Upgrade Kit 2020083
System Upgrade from NovoCyte® 1000 to 2000 2020039
System Upgrade from NovoCyte® 1000 to 2060R 2020040
System Upgrade from NovoCyte® 2000 to 2060R 2020041
System Upgrade from NovoCyte® 2000 to 3000 2020087
System Upgrade from NovoCyte® 2060R to 3000 2020042
System Upgrade from NovoCyte® 1000 to 3000 2020043
System Upgrade from NovoCyte® 1000 to 2070V 2020053
System Upgrade from NovoCyte® 1000 to 2110V 2020054
System Upgrade from NovoCyte® 1000 to 2100YB 2020074
System Upgrade from NovoCyte® 2000 to 3000RYB 2020075
System Upgrade from NovoCyte® 2060 to 3000RYB 2020076
System Upgrade from NovoCyte® 2100YB to 3000VYB 2020077
High Performance Computer Laptop Upgrade 2020084
High Performance Computer Desktop Upgrade 2020085
ACEA NovoExpress® Software, Single License 1010013
ACEA NovoExpress® Software, Site License 1010017
NovoCyte® Non-Functional Display Unit 2010008
NovoSampler® Pro Non-Functional Display Unit 2010055
1-Year Maintenance Kit for NovoCyte® Flow Cytometer Version 1 2030006
6-Month Maintenance Kit for NovoCyte® Flow Cytometer Version 1 2030008
2-Month Maintenance Kit for NovoCyte® Flow Cytometer Version 1 2030009
1-Year Maintenance Kit for NovoCyte® Flow Cytometer Version 2 2030012
6-Month Maintenance Kit for NovoCyte® Flow Cytometer Version 2 2030013
2-Month Maintenance Kit for NovoCyte® Flow Cytometer Version 2 2030014
NovoCyte® QC Particles (2mL; 50 tests) 8000001
Sphero™ 8 Peaks Rainbow Calibration Particles 8000002
Sphero™ 1 Peak Ultra Rainbow Fluorescent Particles 8000003
NovoCyte® Flow Cytometer Starter Kit 8100002
NovoFlow® Sheath Fluid (1X, 10L) 875B601
NovoFlow® Sheath Fluid (100X, 20mL) Box of 10 bottles (20mL/bottle) 871B607
NovoClean® Solution (1X, 500mL) 872B602
NovoClean® Solution (5X; 500mL) 872B609
NovoClean® Solution (5X; 100mL) 871B609
NovoRinse® Solution (1X; 500mL) 872B603
NovoRinse® Solution (5X; 500mL) 872B610
NovoRinse® Solution (5X; 100mL) 871B610

Extended Warranties – Service Contract Options

Note: Each year of extended service warranty includes 1-year Maintenance Kit Catalog No. 2030006

Description Cat. No.
1-Year Extended Service Warranty for NovoCyte® One-Laser System 2040003
2-Year Extended Service Warranty for NovoCyte® One-Laser System 2040004
3-Year extended Service warranty for NovoCyte® One Laser System 2040016
4-Year extended Service warranty for NovoCyte® One Laser System 2040017
5-Year extended Service warranty for NovoCyte® One Laser System 2040018
6-Year extended Service warranty for NovoCyte® One Laser System 2040019
7-Year extended Service warranty for NovoCyte® One Laser System 2040020
1-Year extended Service warranty for NovoCyte® Two Lasers System 2040005
2-Year extended Service warranty for NovoCyte® Two Lasers System 2040006
3-Year extended Service warranty for NovoCyte® Two Lasers System 2040021
4-Year extended Service warranty for NovoCyte® Two Lasers System 2040022
5-Year extended Service warranty for NovoCyte® Two Lasers System 2040023
6-Year extended Service warranty for NovoCyte® Two Lasers System 2040024
7-Year extended Service warranty for NovoCyte® Two Lasers System 2040025
1-Year extended Service warranty for NovoCyte® Three Lasers System 2040007
2-Year extended Service warranty for NovoCyte® Three Lasers System 2040008
3-Year extended Service warranty for NovoCyte® Three Lasers System 2040026
4-Year extended Service warranty for NovoCyte® Three Lasers System 2040027
5-Year extended Service warranty for NovoCyte® Three Lasers System 2040028
6-Year extended Service warranty for NovoCyte® Three Lasers System 2040029
7-Year extended Service warranty for NovoCyte® Three Lasers System 2040030
Preventative Maintenance Service (includes Consumable Materials and Labor for 2 PMs at 6 Months & 1 Year Only) 2040015
Preventative Maintenance Service (includes Consumable Materials and Labor for Single PM Only) 2040037

Replacement Parts and Accessories

Description Cat. No.
Spigot for NovoFlow® Sheath Fluid Refill 2020002
NovoCyte® Fluidics Station 2020003
NovoFlow® Sheath Fluid Container (3L) 2020004
Waste Container (3L) 2020005
NovoRinse® Solution Container (500mL) 2020006
NovoClean® Solution Container (500mL) 2020007
Fluid Container Quick Coupler and Tubing Set 2020008
12 x 75 mm Tube Holder Version A 2020010
12 x 75 mm Tube Holder Version B 2020068
Eppendorf Tube Holder Version A 2020011
Eppendorf Tube Holder Version B 2020069
NovoSampler® Pro 24 Tube Rack 2020079
NovoSampler® Pro Connection Cable 2020078
NovoCyte® Workstation (NovoExpress® Pre-installed) 2020015
NovoCyte® Workstation Monitor 2020016
NovoCyte® Instrument Power Adapter 2020017
NovoCyte® Instrument Power Cord Set 2020044
USB Cable (NovoCyte® Instrument to Workstation) 2020021
DVI Cable (NovoCyte® Instrument to Fluidics Station) 2020022
NovoCyte Decontamination Kit Version 1 2030015
NovoCyte Decontamination Kit Version 2 2030016
NovoCyte® Fluidics Upgrade (V1 to V2) Kit 2020080