NovoCyte Flow Cytometer

The best value benchtop flow cytometer with high flexibility, enhanced sensitivity and resolution.

NovoCyte® is a high performance benchtop flow cytometer designed for all levels of users and all types of laboratories. This budget-friendly instrument is capable of detecting up to 17 parameters with enhanced sensitivity and resolution. The customizable laser and optical configurations of NovoCyte offer a high degree of flexibility while providing complex cell analysis capabilities. The NovoExpress® software facilitates easy and intuitive sample acquisition and analysis. Automation of multiple fluidic functions eliminates cumbersome and time consuming procedures. User hands-on time is minimized by the flexible NovoSampler® Pro which can automatically analyze samples in single tubes, multi-tube racks, or 24-, 48-, or 96-well plates.

View NovoCyte Flow Cytometer Promotions

 


Address the full range of current and future multi-parameter analysis research needs with the NovoCyte flow cytometer. ACEA brings researchers high performance flow cytometry at a low investment cost with the NovoCyte platform.

ACEA offers a system which is:

  • Powerful- up to 17 parameter detection with enhanced sensitivity and resolution.
  • Intuitive – automated instrument maintenance functions and advanced data analysis capabilities for greater usability.
  • Customizable – 1 to 3 laser options, exchangeable filters, multiple sampling options, and flexible analysis formats.

Other important features enhance usability, such as fixed optical alignment, 24-bit detection dynamic ranges with no need for PMT voltage adjustment, accurate volumetric-based cell counting, pressure sensors to monitor fluidic status in real-time, and automatic cleaning and decontamination processes.

ACEA also developed the NovoExpress software, which is intuitive and easy to use. Flexible analysis templates and plotting tools offer enhanced data analysis efficiency.

New NovoCyte Multi-Option Configuration          Click Here

 

Click here to download our Multi-option Configuration Guide.

Configurable Laser Systems

Click here to download the NovoCyte Specification Sheet.

NovoCyte Violet/Blue/Red  Laser Default Systems

NovoCyte Violet/Yellow/Blue and Red/Yellow/Blue Laser Default Systems

NovoCyte Technical Specifications

Specification and performance claims would be based on certain specific experiment conditions.

Optics

Laser ConfigurationSpatially separated beams with 10 x 80 μm elliptical spots
Optical Alignment ProcedureFixed, no operator alignment required
Flow Cell170 x 290 μm rectangular quartz flow cell
Scatter Resolution0.2 μm
Specification and performance claims would be based on certain specific experiment conditions.
Cell Size0.2 – 50 μm
Fluorescence Threshold SensitivityFITC < 75 MESF; PE < 50 MESF; APC <20 MESF
Fluorescence Resolution< 3% CV for CEN
FiltersUser Exchangeable

Fluidics

Sample Acquisition Rate35,000 events/second
Volumetric Absolute Count PrecisionSyringe Pump: CV < 5%
Sample Flow Rate5-120 μL/min
Sheath Flow Rate6.5 mL/min
Sample Aspiration Volume10 μL – 5 mL
Fluid Container Capacity3 L sheath, 3 L waste, 500 mL cleaning, 500 mL decontamination
Carryover< 0.1%
Fluidics MaintenanceAutomated startup, cleaning, decontamination and shutdown

Data Processing

ParametersHeight and Area for FSC, SSC and all Fluorescence Channels, Width and Time
Dynamic Range24 bit; 7.2 decades logarithmic scale; no need for PMT voltage adjustment
CompensationAutomatic compensation, manual compensation, visual compensation tools available for pre/post/live acquisitions
Output Data FilesFCS 3.1, NovoExpress (.ncf), PDF reports, bitmap graphics, vector graphics, CSV
WorkstationDell OptiPlex 7040 SFF or other compatible models, 1 TB with 23.8″ LCD monitor
Computer Operating SystemMicrosoft Windows® 7 Professional (64 bit), Microsoft Office® 2016
SoftwareACEA NovoExpress®

Sampling

Manual Sample Loading12 X 75 mm tube, 1.5 mL Eppendorf tube
Automatic Sample LoadingOptional – compatible with 12 x 75 mm tube, 1.5 & 2 mL tubes, “bullet” tubes in 96-pos. racks, 24-well, 48-well and 96-well microtiter plates

Operating Conditions

Instrument Dimension (W X D X H)23.6 x 17.7 x 15.4 in (60 x 45 x 39 cm)
Instrument Weight86 lb (39 kg)
Power RequirementsInput: 12 VDC, Power: 90 VA
Environment RequirementsTemperature: 15-32˚C ; Relative Humidity: 80% maximum

Optimized PMT Voltage & 7 Decades Detection Dynamic Range

Provides 107 dynamic range for signal detection and processing, offering a broader signal range than other flow cytometers. Wide range of fluorescent and scattering signal intensities eliminates the need for complicated and laborious PMT voltage adjustment. Data acquisition is just load-and-go.

Configurable Fluorescence Detection Channels for Enhanced Assay Flexibility

Customizable selection and upgrade with lasers of different wavelengths allow for personalized, versatile choices of fluorochromes for flow cytometry assays. User interchangeable filters and dichroic mirrors broaden available fluorescence channels to expand users’ detection options.

15 Fluorescent Channel Detection: Multiple Laser Options Providing Flexibility of Choice (405nm, 488nm, 561nm, 640nm)

Customize the instrument to meet detection needs with blue, red and violet laser options. System can be upgraded as analytical requirements increase – no need to purchase another instrument. State-of-the-art solid-state lasers provide high quality and extremely stable optical illumination.

Novel Optical Design & Enhanced Signal Detection

Patent pending independent beam shaping optics and light collection system maximizes signal detection efficiency, increasing sensitivity and resolution for each fluorescence channel. Fixed optical alignment removes need for daily maintenance and adjustment of optical system, providing convenient operation and superior long-term stability. Optimized forward scatter obscuration bar increases sensitivity. Enhanced small particle detection and resolution as a result of optimized obscuration bar design.

Volumetric Fluidics System for Increased Accuracy & Easy Maintenance

The NovoCyte flow cytometer uses a highly accurate, volumetric syringe pump which enables direct absolute counting of samples, eliminating the need to use expensive reference beads.
volumetric-count
Real-time monitoring of fluidics prevents flow path obstruction. Accurate pressure sensors monitor fluidic status in real-time, thus the possibility of flow path obstruction is greatly reduced, allowing for reliable and accurate data acquisition. Automated SIP (sample injection probe) washing following sample acquisition minimizes sample carryover and increases capability to detect rare events. High speed data acquisition of 35,000 events per second.
QC
Routine maintenance features are as simple as clicking a button and following the on screen instructions

High Sensitivity & High Resolution Detection

Highly efficient light collection ensures reliable detection of sub-micron particles and dim signals.

Using innovative optical design for light collection and high quality PMTs, fluorescence signals are efficiently detected with exceptionally high signal-to-noise ratio, ensuring a high detection sensitivity for weak positive samples and small particles.
img_feature2

High Fluorescent Signal Resolution Improves Detection Accuracy

Advanced optical and fluidic system design, premier quality components, and innovative signal processing algorithms collectively ensure accurate extraction of detection parameters with consistently low variation.
Spherotech8

Option for High-Throughput and Automated Analysis of Multiple Samples

The NovoSampler is a modular autosampler, that can be interchanged with the single tube loader in just a couple minutes, is compatible with 12 x 75mm tube, 1.5 & 2mL tubes and 24/48/96 well plates, allowing for versatile loading modes, and increased throughput. Automatic calibration is just the push of a button and this procedure ensures accuracy between different plate types. Simply select your plate, identify your wells or tubes to run, and start running. The NovoSampler will automatically switch between samples, employ mixing or rinsing steps between samples if desired, and complete your run all while you are away.

NovoCyte Spec Sheet



Experiment Analysis Design

Experiment Manager provides a schematic view of the sample analysis being performed.This allows for access to cytometer setting,compensation matrix, report generation, and data analysis. Multiple samples can be analyzed with the same settings by a simple drag-and-drop template function.

Automated & Versatile Data Analysis Functions

Powerful NovoExpress software allows efficient data acquisition, data analysis and report generation. NovoExpress software provides flexible analysis templates and plotting tools, offering enhanced data analysis efficiency.

NovoExpress Download

Download NovoExpress Software Now!

DataAnalysis

Pre & Post Acquisition Compensation

Instantaneous compensation results can be simply achieved by adjustment of the compensation scaling bar on the plot, allowing rapid and accurate compensation, thus eliminating tedious trial and error adjustments of compensation matrix coefficients.
A simple click of a button initiates the automated cleaning and de-contamination process and effectively removes any residual samples in the fluidics path, saving users’ valuable time from laborious manual cleaning and decontamination routines.

QC2

Rapid Import & Export of Data Files


Applications Overview

NovoCyte’s expandable laser capabilities along with configurable optical detection design guarantee scientists the ability to advance research and to explore new applications in various areas of cellular analysis. NovoCyte provides a user friendly software interface enabling fast data acquisition and analysis of various flow cytometric applications.

Apoptosis

Apoptosis, or programmed cell death, is the process by which cells regulate how they die, activating specific pathways that cause the cell to shrink, condense, and eventually be cleared by phagocytosis. This is in contrast to necrotic cell death where cells die uncontrollably and fall apart, which can lead to detrimental effects such as the activation of an immune response. Therefore, apoptotic cells that die in a very orderly fashion limit disruption of nearby cells and tissue.
There are many ways to measure cell death and distinguish it from apoptosis or necrosis. These assays are easily quantified using the NovoCyte flow cytometer due to automatic compensation settings and a wide dynamic range of fluorescence detection which eliminates the need for any PMT voltage adjustments

Cell Cycle Analysis

Normal human somatic cells are diploids containing a constant amount of DNA. During cell cycle progression, DNA synthesis results in a doubling of total DNA content, followed by restoration of the normal DNA content after mitosis. With the NovoCyte flow cytometer, detailed cell cycle analysis can be performed to understand tumor cell differentiation, cell transformation and cell-compound interaction.

 

Cell Cycle Analysis Figure

Cell Signaling

Flow cytometry has traditionally been used to detect extracellular proteins to identify different cell populations. With the advancement of technology and reagents in recent years, we now are able to easily detect intracellular proteins in individual cells. One very useful application for the detection of intracellular proteins is to measure specific cells signaling pathways. These pathways are often altered in disease states, such as cancer, and are now easily detected using flow cytometry providing single cell analysis of these signaling changes.
percp

Jurkat cells were either left in culture(pERK unstim) or stimulated with cell stimulation cocktail for 1hr (pERK +stim).  Cells were washed, fixed and permeablized, followed by staining with anti-phospo-ERK1/2 (T204/Y202) PerCP antibody for 1hr.  Cells were washed and analyzed on the NovoCyte flow cytometer for phospho-ERK levels and compared using a histogram overlay generated with the NovoExpress acquisition and analysis software.

Immunophenotyping

Immunophenotyping quickly identifies candidate cell types, sub-classes and functions. The ACEA NovoCyte flow cytometer can be used for multi-parameter lymphocyte sub-population counting and phenotyping, helper T lymphocyte differentiation and functional study, new sub-population identification, disease specific sub-population identification, and cell surface marker detection.

immunophenotyping

Intracellular Protein Detection

Flow cytometry has traditionally been used to detect extracellular proteins to identify different cell populations. With the advancement of technology and reagents in recent years, we now are able to easily detect intracellular proteins in individual cells. One very useful application for the detection of intracellular proteins is to measure specific cells signaling pathways. These pathways are often altered in disease states, such as cancer, and are now easily detected using flow cytometry providing single cell analysis of these signaling changes.
percp

Jurkat cells were either left in culture(pERK unstim) or stimulated with cell stimulation cocktail for 1hr (pERK +stim).  Cells were washed, fixed and permeablized, followed by staining with anti-phospo-ERK1/2 (T204/Y202) PerCP antibody for 1hr.  Cells were washed and analyzed on the NovoCyte flow cytometer for phospho-ERK levels and compared using a histogram overlay generated with the NovoExpress acquisition and analysis software.

Proliferation

Cell proliferation is an essential function and highly structured event that when unregulated, can cause disease. We can measure proliferation through absolute cell counts or with a dye, such as CFSE. When cells labeled with CFSE divide, the dye is partitioned equally between daughter cells and we can measure the loss of CFSE fluorescence over time as the dye is continuously diluted. The mean fluorescence intensity (MFI) of the dye was also plotted with cell concentration over time to show the inverse relationship between the two. This type of assay is often used to look at changes in T lymphocyte activation.

 

App Note 6 Image

Application Notes

NovoCyte® QC Particles Lot File

Introduction:

1. Download the QC particles lot file corresponding to the lot number of the QC particles.
2. Save the QC particles lot file to the “\QC\QC Beads” folder in the NovoExpress® installation directory ( the default installation directory for NovoExpress is C:\Program Files (x86)\NovoExpress ).
3. Run NovoCyte QC test.

Downloads:

Note: Please upgrade NovoExpress to Version 1.0.2 or newer to use the QC particles lot files (right click on the corresponding lot number, choose "Save Link As", and save to local).

Videos

Live Technical Webinar: Compensation in Flow Cytometry - Date: June 28, 2018 About this Webinar Compensation in flow cytometry can often be challenging and sometimes confusing, however doing compensation correctly is absolutely essential for valid and optimal analyses of multicolor flow cytometry experiments. This webinar will discuss fluorescence spillover, compensation, how to calculate compensation accurately, best practices and considerations for control samples, and some … Continued

Date: June 28, 2018

About this Webinar

Compensation in flow cytometry can often be challenging and sometimes confusing, however doing compensation correctly is absolutely essential for valid and optimal analyses of multicolor flow cytometry experiments. This webinar will discuss fluorescence spillover, compensation, how to calculate compensation accurately, best practices and considerations for control samples, and some challenges that may be encountered. Participants will learn tips and tricks on how to prepare high-quality compensation controls and how to create an optimal compensation matrix, resulting in higher confidence in data analyses and more robust data.

About the Presenter

Heather Paich, Ph.D.
Field Application Scientist
ACEA Biosciences Inc.

Heather Paich received her PhD in Nutritional Biochemistry at the University of North Carolina at Chapel Hill where her research focused on how obesity affects the immune response to pandemic H1N1 influenza. After completing a Fogarty Global Health Post-Doctoral Fellowship in Cape Town, South Africa, where her work focused on infectious diseases, she joined ACEA Biosciences, where she is currently a Senior Field Application Scientist for Flow Cytometry.

A Flow Cytometry Method for Detecting Bacteria in Water - About this Webinar Regulatory testing for microbiological water quality is often conducted by growing microbes on agar medium in visible colonies. However, this is a time-consuming process and not all bacteria can grow in media and therefore be detected using this method. Flow cytometers with a high sensitivity of detection provide tools for detecting and … Continued

About this Webinar

Regulatory testing for microbiological water quality is often conducted by growing microbes on agar medium in visible colonies. However, this is a time-consuming process and not all bacteria can grow in media and therefore be detected using this method. Flow cytometers with a high sensitivity of detection provide tools for detecting and analysing microbes independent of their cultivability and allows precise and rapid determinations of microbial bulk parameters and delivers detailed information on the general microbial state.

During this webinar, we will cover:

1. Advantages of flow cytometry for microbiological water quality testing
2. How to determine absolute counts, nucleic acid content, activity, and classification of bacteria
3. Tips on running a flow cytometry experiment for microbial analysis
4. Data analysis of treated water samples using the NovoExpress software

 

About the Presenter

Stephanie Ting, M. Eng
Field Application Scientist – SE Asia, ANZ, HK&Macau, India,
ACEA Biosciences Inc.

Stephanie received her Masters in Mechanical Engineering from the University of Tokyo, Japan where she studied the effects of mechanobiological stimuli on the production of cartilage-like tissues using mesenchymal stem cells. Currently, she is the Field Application Scientist for SE Asia, ANZ, HK&Macau, and India.

 

WEBINAR – Cell Cycle Analysis by Flow Cytometry -  About this Webinar DNA content analysis using flow cytometry is a powerful tool that can determine the effect of treatments on cell cycle and ploidy in particular relevance to tumours. However, how do you determine which DNA-binding dye to use? How do you accurately differentiate the different phases in the cell cycle in your … Continued

About this Webinar

DNA content analysis using flow cytometry is a powerful tool that can determine the effect of treatments on cell cycle and ploidy in particular relevance to tumours. However, how do you determine which DNA-binding dye to use? How do you accurately differentiate the different phases in the cell cycle in your population of cells? This webinar will teach the methods for cell cycle analysis with the following topics:

During this webinar, we will cover:

  • Phases of a typical mammalian cell cycle
  • Characteristics of Nucleic Acid Stains
  • How to choose the right fluorochromes and properly prepare your cells
  • DNA ploidy & tumours

Session One:

Date: Thursday, December 21, 2017
Time: 8 AM SINGAPORE | 9 AM TOKYO | 4 PM PST (Dec. 20)

Session Two:

Date: Thursday, December 21, 2017
Time: 7 AM PST | 10 AM EST | 4 PM PARIS

 

About the Presenter

Stephanie Ting, M. Eng
Field Application Scientist – SE Asia, ANZ, HK&Macau, India,
ACEA Biosciences Inc.

Stephanie received her Masters in Mechanical Engineering from the University of Tokyo, Japan where she studied the effects of mechanobiological stimuli on the production of cartilage-like tissues using mesenchymal stem cells. Currently, she is the Field Application Scientist for SE Asia, ANZ, HK&Macau, and India.

 

 

WEBINAR – Measuring Calcium Flux by Flow Cytometry - Accurate measurements of intracellular Ca2+ concentrations allows for a more comprehensive understanding of Ca2+ regulated cell functions and signaling pathways. Ca2+ is an important molecule that affects a wide range of physiological and pathological processes.

Accurate measurements of intracellular Ca2+ concentrations allows for a more comprehensive understanding of Ca2+ regulated cell functions and signaling pathways. Ca2+ is an important molecule that affects a wide range of physiological and pathological processes. Unlike other secondary messengers, Ca2+ is not synthesized or metabolized, but stored and rapidly released by channels/pumps that maintain Ca2+ concentrations in distinct cellular compartments. Flow cytometry allows real time measurement of a calcium flux response utilizing fluorescent Ca2+ indicators which exhibit an increase in fluorescence upon binding to Ca2+. In this webinar we will discuss the basics of calcium flux measurements by flow cytometry, providing specific details on experiment setup, acquisition, and analysis on the NovoCyte flow cytometer.

Key topics to be covered:
1. Essentials of calcium measurements by flow cytometer
2. Calcium flux experimental setup and acquisition on the NovoCyte
3. Detailed analysis of calcium flux in a T cell line using calcium indicator dye Fluo-4/AM

Learn More about the NovoCyte Flow Cytometer       Click here

Speaker:

Lauren Jachimowicz, Ph.D. 
Application Development Scientist, ACEA Biosciences, Inc.
Lauren Jachimowicz received her PhD in Biological Science from the University of California, San Diego where she studied the role of the circadian clock genes in T cells. Currently, she is the Application Development Scientist for flow cytometry at ACEA Biosciences advancing flow cytometry into new fields of research.

WEBINAR: Flow Cytometric Assessment, Quantification and Regulation of Human Neutrophil Extracellular Traps (NETS) - At the CYTO 2017 meeting Dr. Adriano Rossi and Dr. Calum Robb from the University of Edinburgh described the development of a new flow cytometry-based assay for studying NETosis, a cell death process that occurs when stimulated neutrophils cast an extracellular web containing DNA, histones, and anti-bacterial proteins that ensnare/neutralize invading organisms. Using the NovoCyte® … Continued


At the CYTO 2017 meeting Dr. Adriano Rossi and Dr. Calum Robb from the University of Edinburgh described the development of a new flow cytometry-based assay for studying NETosis, a cell death process that occurs when stimulated neutrophils cast an extracellular web containing DNA, histones, and anti-bacterial proteins that ensnare/neutralize invading organisms. Using the NovoCyte® flow cytometer, they demonstrate the detection and quantification of neutrophil extracellular traps (NETs), as well as analysis of the regulatory mechanisms involved.

WEBINAR: Flow Cytometry: Simplifying Assay Panel Design and WorkFLOW - About this Webinar Researchers and flow cytometry users spend a significant amount of time designing flow assay panels and analyzing their samples. In this webinar, viewers will have the chance to learn about  tools and recent developments in flow cytometry  that simplify assay design and workflow. Our speakers will explore the capabilities of benchtop and … Continued

About this Webinar

Researchers and flow cytometry users spend a significant amount of time designing flow assay panels and analyzing their samples.

In this webinar, viewers will have the chance to learn about  tools and recent developments in flow cytometry  that simplify assay design and workflow. Our speakers will explore the capabilities of benchtop and cost-effective flow cytometry systems and the easiest way to design flow panels.

Webinar Features

  • Design and capabilities of a NovoCyte flow cytometer
  • New features that simplify setup, acquisition, and analysis
  • How to design multicolor fluorescent panels using FluoroFinder’s experiment design platform
  • Tips for building better panels in less time

Featured Speakers:

Garret Guenther, Ph.D.
ACEA Biosciences.

Jeff Jensen, Ph.D.
FluoroFinder, LLC

 

NovoCyte Webinar Series – Measuring Apoptosis by Flow Cytometry - Apoptosis, or programmed cell death, is a tightly regulated cellular phenomenon which includes many different signaling and effector pathways. By interrogating these processes in individual cells, and by evaluating large numbers of cells rapidly, flow cytometry can efficiently

WEBINAR – Flow Cytometry: Emerging Instrumentation and Application Trends | Wed, Feb 15, 2017 - Flow cytometry remains an essential tool for cell analysis. Although often relegated to specialized core facilities, flow cytometry instruments are in fact becoming increasingly commonplace in life science labs. In this special webinar, viewers will have the chance to learn about recent developments in flow cytometry technology, including the emergence of lower cost, smaller flow cytometry options with the capability of analyzing multiple … Continued

Flow cytometry remains an essential tool for cell analysis. Although often relegated to specialized core facilities, flow cytometry instruments are in fact becoming increasingly commonplace in life science labs.

In this special webinar, viewers will have the chance to learn about recent developments in flow cytometry technology, including the emergence of lower cost, smaller flow cytometry options with the capability of analyzing multiple fluorescent colors. Our speakers will explore the design, development, and uses for smaller, more cost-effective flow cytometry systems in research labs. In this webinar, you will gain insights on:

  • Design and development of the latest multicolor flow cytometry instruments
  • Application of flow cytometry techniques for different experimental needs
  • How analyzing multiple cellular parameters using different  fluorescent colors can extend experimental possibilities
  • How to effectively analyze and interpret your flow cytometry data sets
Learn More about the NovoCyte Flow Cytometer       Click here

Speakers:

Paul K. Wallace, PhD
Roswell Park Cancer Institute
Paul Wallace has served since 2003 as Director of the Flow and Image Cytometry Department at the Roswell Park Cancer Institute (RPCI) in Buffalo, NY, where he is a Professor of Oncology. He is also an Associate Professor of Biotechnical and Clinical Laboratory Sciences at the University at Buffalo. His primary expertise and board certification is in the flow cytometric diagnosis of hematological malignancies.

Christopher Groves 
MedImmune, Inc. 
Christopher Groves is a Senior Manager at MedImmune Inc., where he heads a keystone technology group  challenged with expanding knowledge of disease mechanisms while also delivering innovative solutions in drug discovery and manufacturing that provide scientists access to cutting-edge technological capability.

William Telford, PhD 
Experimental Transplantation and Immunology Branch, NCI-NIH 
William Telford is the Head of the NCI-NIH Flow Cytometry core facility, which provides state-of-the-art flow cytometry as well as imaging technology and instrumentation to users in the research community.

Moderator:

Patrick C.H. Lo, PhD 
Senior Editor, BioTechniques

Videos: Research Presentations

Live Technical Webinar: Compensation in Flow Cytometry - Date: June 28, 2018 About this Webinar Compensation in flow cytometry can often be challenging and sometimes confusing, however doing compensation correctly is absolutely essential for valid and optimal analyses of multicolor flow cytometry experiments. This webinar will discuss fluorescence spillover, compensation, how to calculate compensation accurately, best practices and considerations for control samples, and some … Continued

Date: June 28, 2018

About this Webinar

Compensation in flow cytometry can often be challenging and sometimes confusing, however doing compensation correctly is absolutely essential for valid and optimal analyses of multicolor flow cytometry experiments. This webinar will discuss fluorescence spillover, compensation, how to calculate compensation accurately, best practices and considerations for control samples, and some challenges that may be encountered. Participants will learn tips and tricks on how to prepare high-quality compensation controls and how to create an optimal compensation matrix, resulting in higher confidence in data analyses and more robust data.

About the Presenter

Heather Paich, Ph.D.
Field Application Scientist
ACEA Biosciences Inc.

Heather Paich received her PhD in Nutritional Biochemistry at the University of North Carolina at Chapel Hill where her research focused on how obesity affects the immune response to pandemic H1N1 influenza. After completing a Fogarty Global Health Post-Doctoral Fellowship in Cape Town, South Africa, where her work focused on infectious diseases, she joined ACEA Biosciences, where she is currently a Senior Field Application Scientist for Flow Cytometry.

A Flow Cytometry Method for Detecting Bacteria in Water - About this Webinar Regulatory testing for microbiological water quality is often conducted by growing microbes on agar medium in visible colonies. However, this is a time-consuming process and not all bacteria can grow in media and therefore be detected using this method. Flow cytometers with a high sensitivity of detection provide tools for detecting and … Continued

About this Webinar

Regulatory testing for microbiological water quality is often conducted by growing microbes on agar medium in visible colonies. However, this is a time-consuming process and not all bacteria can grow in media and therefore be detected using this method. Flow cytometers with a high sensitivity of detection provide tools for detecting and analysing microbes independent of their cultivability and allows precise and rapid determinations of microbial bulk parameters and delivers detailed information on the general microbial state.

During this webinar, we will cover:

1. Advantages of flow cytometry for microbiological water quality testing
2. How to determine absolute counts, nucleic acid content, activity, and classification of bacteria
3. Tips on running a flow cytometry experiment for microbial analysis
4. Data analysis of treated water samples using the NovoExpress software

 

About the Presenter

Stephanie Ting, M. Eng
Field Application Scientist – SE Asia, ANZ, HK&Macau, India,
ACEA Biosciences Inc.

Stephanie received her Masters in Mechanical Engineering from the University of Tokyo, Japan where she studied the effects of mechanobiological stimuli on the production of cartilage-like tissues using mesenchymal stem cells. Currently, she is the Field Application Scientist for SE Asia, ANZ, HK&Macau, and India.

 

WEBINAR – Cell Cycle Analysis by Flow Cytometry -  About this Webinar DNA content analysis using flow cytometry is a powerful tool that can determine the effect of treatments on cell cycle and ploidy in particular relevance to tumours. However, how do you determine which DNA-binding dye to use? How do you accurately differentiate the different phases in the cell cycle in your … Continued

About this Webinar

DNA content analysis using flow cytometry is a powerful tool that can determine the effect of treatments on cell cycle and ploidy in particular relevance to tumours. However, how do you determine which DNA-binding dye to use? How do you accurately differentiate the different phases in the cell cycle in your population of cells? This webinar will teach the methods for cell cycle analysis with the following topics:

During this webinar, we will cover:

  • Phases of a typical mammalian cell cycle
  • Characteristics of Nucleic Acid Stains
  • How to choose the right fluorochromes and properly prepare your cells
  • DNA ploidy & tumours

Session One:

Date: Thursday, December 21, 2017
Time: 8 AM SINGAPORE | 9 AM TOKYO | 4 PM PST (Dec. 20)

Session Two:

Date: Thursday, December 21, 2017
Time: 7 AM PST | 10 AM EST | 4 PM PARIS

 

About the Presenter

Stephanie Ting, M. Eng
Field Application Scientist – SE Asia, ANZ, HK&Macau, India,
ACEA Biosciences Inc.

Stephanie received her Masters in Mechanical Engineering from the University of Tokyo, Japan where she studied the effects of mechanobiological stimuli on the production of cartilage-like tissues using mesenchymal stem cells. Currently, she is the Field Application Scientist for SE Asia, ANZ, HK&Macau, and India.

 

 

WEBINAR – Measuring Calcium Flux by Flow Cytometry - Accurate measurements of intracellular Ca2+ concentrations allows for a more comprehensive understanding of Ca2+ regulated cell functions and signaling pathways. Ca2+ is an important molecule that affects a wide range of physiological and pathological processes.

Accurate measurements of intracellular Ca2+ concentrations allows for a more comprehensive understanding of Ca2+ regulated cell functions and signaling pathways. Ca2+ is an important molecule that affects a wide range of physiological and pathological processes. Unlike other secondary messengers, Ca2+ is not synthesized or metabolized, but stored and rapidly released by channels/pumps that maintain Ca2+ concentrations in distinct cellular compartments. Flow cytometry allows real time measurement of a calcium flux response utilizing fluorescent Ca2+ indicators which exhibit an increase in fluorescence upon binding to Ca2+. In this webinar we will discuss the basics of calcium flux measurements by flow cytometry, providing specific details on experiment setup, acquisition, and analysis on the NovoCyte flow cytometer.

Key topics to be covered:
1. Essentials of calcium measurements by flow cytometer
2. Calcium flux experimental setup and acquisition on the NovoCyte
3. Detailed analysis of calcium flux in a T cell line using calcium indicator dye Fluo-4/AM

Learn More about the NovoCyte Flow Cytometer       Click here

Speaker:

Lauren Jachimowicz, Ph.D. 
Application Development Scientist, ACEA Biosciences, Inc.
Lauren Jachimowicz received her PhD in Biological Science from the University of California, San Diego where she studied the role of the circadian clock genes in T cells. Currently, she is the Application Development Scientist for flow cytometry at ACEA Biosciences advancing flow cytometry into new fields of research.

WEBINAR: Flow Cytometric Assessment, Quantification and Regulation of Human Neutrophil Extracellular Traps (NETS) - At the CYTO 2017 meeting Dr. Adriano Rossi and Dr. Calum Robb from the University of Edinburgh described the development of a new flow cytometry-based assay for studying NETosis, a cell death process that occurs when stimulated neutrophils cast an extracellular web containing DNA, histones, and anti-bacterial proteins that ensnare/neutralize invading organisms. Using the NovoCyte® … Continued


At the CYTO 2017 meeting Dr. Adriano Rossi and Dr. Calum Robb from the University of Edinburgh described the development of a new flow cytometry-based assay for studying NETosis, a cell death process that occurs when stimulated neutrophils cast an extracellular web containing DNA, histones, and anti-bacterial proteins that ensnare/neutralize invading organisms. Using the NovoCyte® flow cytometer, they demonstrate the detection and quantification of neutrophil extracellular traps (NETs), as well as analysis of the regulatory mechanisms involved.

WEBINAR: Flow Cytometry: Simplifying Assay Panel Design and WorkFLOW - About this Webinar Researchers and flow cytometry users spend a significant amount of time designing flow assay panels and analyzing their samples. In this webinar, viewers will have the chance to learn about  tools and recent developments in flow cytometry  that simplify assay design and workflow. Our speakers will explore the capabilities of benchtop and … Continued

About this Webinar

Researchers and flow cytometry users spend a significant amount of time designing flow assay panels and analyzing their samples.

In this webinar, viewers will have the chance to learn about  tools and recent developments in flow cytometry  that simplify assay design and workflow. Our speakers will explore the capabilities of benchtop and cost-effective flow cytometry systems and the easiest way to design flow panels.

Webinar Features

  • Design and capabilities of a NovoCyte flow cytometer
  • New features that simplify setup, acquisition, and analysis
  • How to design multicolor fluorescent panels using FluoroFinder’s experiment design platform
  • Tips for building better panels in less time

Featured Speakers:

Garret Guenther, Ph.D.
ACEA Biosciences.

Jeff Jensen, Ph.D.
FluoroFinder, LLC

 

NovoCyte Webinar Series – Measuring Apoptosis by Flow Cytometry - Apoptosis, or programmed cell death, is a tightly regulated cellular phenomenon which includes many different signaling and effector pathways. By interrogating these processes in individual cells, and by evaluating large numbers of cells rapidly, flow cytometry can efficiently

WEBINAR – Flow Cytometry: Emerging Instrumentation and Application Trends | Wed, Feb 15, 2017 - Flow cytometry remains an essential tool for cell analysis. Although often relegated to specialized core facilities, flow cytometry instruments are in fact becoming increasingly commonplace in life science labs. In this special webinar, viewers will have the chance to learn about recent developments in flow cytometry technology, including the emergence of lower cost, smaller flow cytometry options with the capability of analyzing multiple … Continued

Flow cytometry remains an essential tool for cell analysis. Although often relegated to specialized core facilities, flow cytometry instruments are in fact becoming increasingly commonplace in life science labs.

In this special webinar, viewers will have the chance to learn about recent developments in flow cytometry technology, including the emergence of lower cost, smaller flow cytometry options with the capability of analyzing multiple fluorescent colors. Our speakers will explore the design, development, and uses for smaller, more cost-effective flow cytometry systems in research labs. In this webinar, you will gain insights on:

  • Design and development of the latest multicolor flow cytometry instruments
  • Application of flow cytometry techniques for different experimental needs
  • How analyzing multiple cellular parameters using different  fluorescent colors can extend experimental possibilities
  • How to effectively analyze and interpret your flow cytometry data sets
Learn More about the NovoCyte Flow Cytometer       Click here

Speakers:

Paul K. Wallace, PhD
Roswell Park Cancer Institute
Paul Wallace has served since 2003 as Director of the Flow and Image Cytometry Department at the Roswell Park Cancer Institute (RPCI) in Buffalo, NY, where he is a Professor of Oncology. He is also an Associate Professor of Biotechnical and Clinical Laboratory Sciences at the University at Buffalo. His primary expertise and board certification is in the flow cytometric diagnosis of hematological malignancies.

Christopher Groves 
MedImmune, Inc. 
Christopher Groves is a Senior Manager at MedImmune Inc., where he heads a keystone technology group  challenged with expanding knowledge of disease mechanisms while also delivering innovative solutions in drug discovery and manufacturing that provide scientists access to cutting-edge technological capability.

William Telford, PhD 
Experimental Transplantation and Immunology Branch, NCI-NIH 
William Telford is the Head of the NCI-NIH Flow Cytometry core facility, which provides state-of-the-art flow cytometry as well as imaging technology and instrumentation to users in the research community.

Moderator:

Patrick C.H. Lo, PhD 
Senior Editor, BioTechniques

Instrument – Includes Workstation & NovoExpress Software

ModelLaser ConfigurationDescriptionCat. No.
ACEA NovoCyte® 1000Blue Laser (488 nm)3 color, FSC, SSC2010001
ACEA NovoCyte® 1040 4FL ChannelsBlue Laser (530/572/675/780 nm)4 color, FSC, SSC2010034
ACEA NovoCyte® 1050 5 FL ChannelsBlue Laser (530/572/615/675/780 nm)5 color, FSC, SSC2010035
ACEA NovoCyte® 2000RBlue / Red Laser (488/640 nm)4 color, FSC, SSC2010002
ACEA NovoCyte® 2060RBlue / Red Laser (488/640 nm)6-7 color, FSC, SSC2010004
ACEA NovoCyte® 2070RBlue/Red Laser (488/640 nm)7-8 Color, FSC, SSC2010065
ACEA NovoCyte® 2070V 7 FL ChannelsBlue / Violet Laser (445/530/572/695 nm)7 color, FSC, SSC2010025
ACEA NovoCyte® 2090VBlue/Violet Laser (488/405 nm)9 Color, FSC, SSC2010067
ACEA NovoCyte® 2110V 11 FL ChannelsBlue / Violet Laser (445/530/572/660/695/780 nm)11 color, FSC, SSC2010027
ACEA NovoCyte® 3000Violet/Blue/Red Laser (488/640/405 nm)13 color, FSC, SSC2010011
ACEA NovoCyte® 3005Violet/Blue/Red Laser (405/488/640 nm)14-15 Color, FSC, SSC2010064
ACEA NovoCyte® 3000RYBRed / Yellow / Blue Laser (640/561/488 nm)13-14 color, FSC, SSC2010041
ACEA NovoCyte® 3000VYBViolet / Yellow / Blue Laser (405/561/488 nm)14 color, FSC, SSC2010042
ACEA NovoCyte® 2100YBYellow / Blue Laser (561/488 nm)10 color, FSC, SSC2010043
ACEA NovoCyte® 1000YYellow Laser (561 nm)1 color, FSC, SSC2010001
ACEA NovoCyte® 1000 and NovoSampler® Pro ComboBlue Laser (488 nm)3 color, FSC, SSC2010047
ACEA NovoCyte® 2000 and NovoSampler® Pro ComboBlue / Red Laser (488/640 nm)4 color, FSC, SSC2010048
ACEA NovoCyte® 2060 and NovoSampler® Pro ComboBlue / Red Laser (488/640 nm)6 color, FSC, SSC2010049
ACEA NovoCyte® 3000 and NovoSampler® Pro ComboBlue / Red / Violet Laser (488/640/405 nm)13 color, FSC, SSC2010050
ACEA NovoCyte® 3000RYB and NovoSampler® Pro ComboRed / Yellow / Blue Laser (640/561/488 nm)13 color, FSC, SSC2010044
ACEA NovoCyte® 3000VYB and NovoSampler® Pro ComboViolet / Yellow / Blue Laser (405/561/488 nm)13 color, FSC, SSC2010045
ACEA NovoCyte® 2100YB and NovoSampler® Pro ComboYellow / Blue Laser (561/488 nm)10 color, FSC, SSC2010046

 

DescriptionCat. No.
ACEA NovoSampler® Pro2020070
NovoCyte® SIP Upgrade Kit2020083
System Upgrade from NovoCyte® 1000 to 20002020039
System Upgrade from NovoCyte® 1000 to 2060R2020040
System Upgrade from NovoCyte® 2000 to 2060R2020041
System Upgrade from NovoCyte® 2000 to 30002020087
System Upgrade from NovoCyte® 2060R to 30002020042
System Upgrade from NovoCyte® 1000 to 30002020043
System Upgrade from NovoCyte® 1000 to 2070V2020053
System Upgrade from NovoCyte® 1000 to 2110V2020054
System Upgrade from NovoCyte® 1000 to 2100YB2020074
System Upgrade from NovoCyte® 2000 to 3000RYB2020075
System Upgrade from NovoCyte® 2060 to 3000RYB2020076
System Upgrade from NovoCyte® 2100YB to 3000VYB2020077
System Upgrade from NovoCyte 2060 to 3005 2020096

Workstation and Software

DescriptionCat. No.
High Performance Computer Laptop Upgrade2020084
High Performance Computer Desktop Upgrade2020085
ACEA NovoExpress® Software, Single License1010013
ACEA NovoExpress® Software, Site License1010017
NovoCyte® Non-Functional Display Unit2010008
NovoSampler® Pro Non-Functional Display Unit2010055

Filters and Mirrors

DescriptionCat. No.
Bandpass Filter Assembly 445/45 nm2050015
Bandpass Filter Assembly 488/10 nm2060121
Bandpass Filter Assembly 512/25 nm2060140
Bandpass Filter Assembly 530/30 nm2060120
Bandpass Filter Assembly 550/49 nm2060139
Bandpass Filter Assembly 561/14 nm2060118
Bandpass Filter Assembly 572/28 nm2060119
Bandpass Filter Assembly 586/20 nm2060117
Bandpass Filter Assembly 590/36 nm2060138
Bandpass Filter Assembly 615/20 nm2050011
Bandpass Filter Assembly 630/30 nm2060137
Bandpass Filter Assembly 660/20 nm2060091
Bandpass Filter Assembly 675/30 nm2060092
Bandpass Filter Assembly 695/40 nm2060089
Bandpass Filter Assembly 725/40 nm2060116
Bandpass Filter Assembly 780/60 nm2050018
Dichroic Mirror Assembly 472 nm Short Pass (SP)2050012
Dichroic Mirror Assembly 495 nm Long Pass (LP)2060128
Dichroic Mirror Assembly 505 nm Long Pass (LP)2060127
Dichroic Mirror Assembly 555 nm Long Pass (LP)2060126
Dichroic Mirror Assembly 572 nm Long Pass (LP)2060125
Dichroic Mirror Assembly 600 nm Long Pass (LP)2050013
Dichroic Mirror Assembly 650 nm Long Pass (LP)2060124
Dichroic Mirror Assembly 685 nm Long Pass (LP)2060090
Dichroic Mirror Assembly 705 nm Long Pass (LP)2060123
Dichroic Mirror Assembly 735 nm Long Pass (LP)2050014
Dichroic Mirror Assembly 757 nm Long Pass (LP)2060122
Dichroic Mirror Assembly 705 nm Long Pass (LP)2060123
Dichroic Mirror Assembly 735 nm Long Pass (LP)2050014
Dichroic Mirror Assembly 757 nm Long Pass (LP)2060122

Reagents and Consumables

DescriptionCat. No.
NovoCyte® QC Particles8000001
Sphero™ 8 Peaks Rainbow Calibration Particles8000002
Sphero™ 1 Peak Ultra Rainbow Fluorescent Particles8000003
NovoFlow® Sheath Fluid (1X, 10L)875B601
NovoClean® Solution (1X, 500mL)872B602
NovoClean® Solution (5X, 500mL)872B609
NovoClean® Solution (5X, 100mL)871B609
NovoRinse® Solution (1X, 500mL)872B603
NovoRinse® Solution (5X, 500mL)872B610
NovoRinse® Solution (5X, 100mL)871B610
NovoFlow® Solution (100X, 20mL x 10 bottles)871B607
NovoCyte® Flow Cytometer Starter Kit Version 28100002

Maintenance Parts and Consumables

DescriptionCat. No.
NovoFlow® Sheath Fluid Inlet Filter2030001
NovoFlow® Sheath Fluid In-line Filter2030002
Waste Filter2030003
Peristaltic Pump Tubing2030004
NovoRinse® Solution Inlet Filter2030005
NovoClean® Solution Inlet Filter2030007
1-Year Fluidic System Maintenance Kit Version 12030006
6-Month Fluidic System Maintenance Kit Version 12030008
2-Months Fluidic System Maintenance Kit Version 12030009
1-Year Fluidic System Maintenance Kit Version 22030012
6-Month Fluidic System Maintenance Kit Version 22030013
2-Month Fluidic System Maintenance Kit Version 22030014
Tubing Kit for Instrument Internal Fluidics Version 12060073
Tubing Kit for Instrument Internal Fluidics Version 22060080

Replacement Parts and Accessories

DescriptionCat. No.
Spigot for NovoFlow® Sheath Fluid Refill2020002
NovoCyte® Fluidics Station2020003
NovoFlow® Sheath Fluid Container (3L)2020004
Waste Container (3L)2020005
NovoRinse® Solution Container (500mL)2020006
NovoClean® Solution Container (500mL)2020007
Fluid Container Quick Coupler and Tubing Set2020008
12 x 75 mm Tube Holder Version A2020010
12 x 75 mm Tube Holder Version B2020068
Eppendorf Tube Holder Version A2020011
Eppendorf Tube Holder Version B2020069
NovoSampler® Pro 24 Tube Rack2020079
NovoSampler® Pro Connection Cable2020078
NovoCyte® Workstation (NovoExpress® Pre-installed)2020015
NovoCyte® Workstation Monitor2020016
NovoCyte® Instrument Power Adapter2020017
NovoCyte® Instrument Power Cord Set2020044
USB Cable (NovoCyte® Instrument to Workstation)2020021
DVI Cable (NovoCyte® Instrument to Fluidics Station)2020022
NovoCyte® Decontamination Kit Version 12030015
NovoCyte® Decontamination Kit Version 22030016
NovoCyte® Fluidics Upgrade (V1 to V2) Kit2020080
NovoCyte® Instrument Transport Case2020050
Work Station Transport Case2020051