Stem Cell and Cell Differentiation

ACEA Biosciences xCELLigence

Real-Time Monitoring and Image Analysis of Human Skin-Derived Precursor (SKPs) Cell Differentiation

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Differentiation of human skin-derived precursors (SKPs) into smooth muscle cells (SMCSs) and undifferentiated SKPs (C) with or without the application of transforming growth factor (TGF)-β1 (A) or TGF-β3(B) and subsequently fixed and stained to display a-smooth actin, calponin, and SM22a. Real-time analysis correlates the increase in Cell Index with that of morphological differences between the differentiated and undifferentiated SKPs (D). (Data and figures adapted from Steinbach SK, et. al., 2011).

Morphological Changes of Keratinocytes During Terminal Differentiation

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(A) Calcium-dependent morphological changes of keratinocytes during terminal differentiation can be exhibited using the RTCA xCELLigence system. (B) The increase in Cell Index correlates with image-based morphological analysis. (Data and figures adapted from Spörl F, et. al., 2010).

Key Benefits of Using xCELLigence for Studying Stem Cells and Cell Differentiation:
  1. Real time data reveals the kinetics of cell differentiation.
  2. Label-free nature minimizes the need for staining with fluorescent markers involving multiple handling steps for fixation and staining.
  3. The non-invasive nature of the assay, in combination with the E-Plate VIEW area allows for correlation of real time data with imaging assays.
Stem Cells and Cell Differentiation Supporting Information:

  • Stem Cells and Cell Differentiation Publications
  1. Optimization and scale-up culture of human endometrial multipotent mesenchymal stromal cells: potential for clinical application. Rajaraman G, White J, Tan KS, Ulrich D, Rosamilia A, Werkmeister J, Gargett CE. Tissue Engineering Part C Methods. 2013 Jan;19(1):80–92.
  2. Comparison of long-term retinoic acid-based neural induction methods of bone marrow in human mesenchymal stem cells. Mammadov B, Karakas N, Isik S. In Vitro Cellular and Developmental Biology. 2011 Aug;47(7):484-91.
  3. Directed differentiation of skin-derived precursors into functional vascular smooth muscle cells. Steinbach SK, El-Mounayri O, DaCosta RS, Frontini MJ, Nong Z, Maeda A, Pickering JG,Miller FD, Husain M. Arteriosclerosis, Thrombosis, and Vascular Biol. 2011 Dec;31(12):2938-48.
  4. Real-time monitoring of membrane cholesterol reveals new insights into epidermal differentiation. Spörl F, Wunderskirchner M, Ullrich O, Bömke G, Breitenbach U, Blatt T, Wenck H, Wittern KP, Schrader A. Journal of Investigative Dermatology. 2010 May;130(5):1268-78.