Regenerative Medicine

Regenerative medicine has the potential to significantly improve healthcare through the engineering of human cells, tissues or organs to restore normal function. To meet this growing demand, new technologies are required to accurately assess the function and quality of these cell-based products for large scale production.

Cell Therapy Manufacturing Challenges

One of the biggest hurdles in manufacturing of cellular therapies using mesenchymal stem cells (MSCs) is the lack of quantitative tools that simultaneously assess quality and potency of MSCs, which display big variations among donors and over limited passages. To generate MSCs for clinical studies, cells from donors must be expanded over several passages in vitro. Because successive passages reduce proliferation rates and cause loss of differentiation potential, and because there is high variability in functional capacity from one donor to the next, it is critical to assess the function of cells both during and after the expansion process.

Benefits of xCELLigence Real Time Cell Analysis for Studying Mesenchymal Stem cells for Cellular Therapies

  • Continuously measure integrated changes in cell number, attachment, and morphology under physiologically relevant conditions to ensure consistency between batches
  • Maximize production yields by using less cells, which can be analyzed at any point during differentiation
  • More effectively predict BMSC functional capacity (i.e. differentiation potential) compared to traditional methods
  • Consistently measure minute changes in cellular morphology as a result of ligand-dependent GPCR activation.
  • Rapidly predict functional status by taking advantage of unique GPCR profiles in undifferentiated versus differentiated BMSCs

 


Applications

Real-Time Quality Control and Functional Assessment of Mesenchymal Stem Cells for Cellular Therapies Real-Time Monitoring of G-Protein Coupled Receptor Activation as a Functional Assessment of Mesenchymal Stem CellsStem Cells and Cell Differentiation