Estrogen Specific Response Monitored on xCELLigence System
The estrogen receptor agonist 17β-estradiol (E2) induced a unique kinetic response profile with delayed cell index growth in the human breast cancer cell line T-47D. Such response was abolished by the estrogen receptor antagonist ICI182780 (A). The progesterone antagonist mifepristone cannot abolish E2 response (B), but rather had a synergistic effect with E2 on T-47D cells. (Data and figures adapted from Jin C, et. al., 2011).
Progesterone Specific Response Monitored on xCELLigence System
Progesterone induced unique kinetic response profile with a biphasic cell index curve in the human breast cancer cell line T-47D. This response was blocked by the progesterone antagonist mifepristone (A), but could not be abolished by the estrogen receptor antagonist ICI182780 (B). (Data and figures adapted from Jin C, et. al., 2011).
Key Benefits of Using xCELLigence for Studying Nuclear Hormone Receptors:
- Label-free nature allows for sensitive detection of endogenous receptor activity.
- Kinetic response profiles may be diagnostic for specific pathways.
- Ability to differentiate cytotoxicity and proliferation with a single experiment.
- Real time data can identify the optimal time to monitor different ligand effects with standard assay.