Immunophenotyping

Immune status is associated with disease state, treatment efficiency, and response to external stimuli such as vaccines. Immunophenotyping quickly identifies candidate cell types, sub-classes and functions. Monitoring the frequency of numerous immune cell population as well as the differentiation/activation status of specific cell subsets such as monocytes, NK cells, T and B cells is essential as they may influence the immunogenicity of a vaccine and its efficiency. The ACEA NovoCyte Flow Cytometer enables simultaneous quantification of multiple leukocytes for better understanding the immune status of patients and surveillance of the immune response to infectious disease.

Based on Optimized Multicolor Immunofluorescence Panel OMIP-024, an 18-color immunophenotyping panel was designed for studying human peripheral blood mononuclear cells (PBMCs) using the NovoCyte QuanteonTM. In this 18-color immunophenotyping stain, the frequency of monocytes, B cells, plasmablasts, CD4+ and CD8+ T cells, regulatory T cells, γδ T cells, NK T cells, NK cells, and dendritic cells was examined. NK and T cell status are indicators of a proper immune response to a vaccine, therefore