Kinetic Response Profiles Reflect Different Cytotoxic Mechanisms
HepG2 cells were treated with various cytotoxic compounds and monitored in real time for cytotoxic response kinetics. Representative compounds targeting tubulin (paclitaxel), actin (cytochalasin D), DNA (doxorubicin), mitochondria (rotenone), calcium homeostasis (thapsigargin), and protein transport (brefeldin A) generated distinct response profiles. (Data and figure adapated from ACEA Biosciences, Inc. unpublished data).
Real Time Monitoring Allows Identification of Optimal Timepoints for Further Analysis
HeLa cells were treated with cytotoxic compounds and monitored in real time for cytotoxic response kinetics on xCELLigence. At the indicated timepoints, cells in a parallel plate were analyzed using the WST-1 cell viability assay. 5-FU or MG132 caused different dose-dependant kinetic response profiles with different optimal times for performing the WST-1 assay. (Data and figure adapted from Ke N, et. al., 2011).
Key Benefits of Using xCELLigence for Studying Compound-mediated Cytotoxicity:
- Kinetic responses can be predictive of mechanism of action.
- Continuous monitoring ensures no meaningful time points are missed.
- Real-time data allows identification of the optimal times for treatment and data collection.
- Non-invasive assay is performed in tissue culture incubator, allowing for analysis by standard viability assays at any point during the experiment.
- Easy quantification of the onset and kinetics of the cytotoxic response.