Quantitative Assessment of Extracellular Matrix Effect on A549 Attachment and Spreading
The Cell Index increases proportionately as the coating concentration of collagen IV increases. (Data and figures adapted from ACEA Biosciences, unpublished data).
Mammary Stem-Like Cells Have Increased Tubulin-Dependent Initial Reattachment From Suspension
(A) Stem-like HMLE cells attach at signifi cantly faster rates than non-stem-like HMLE cells as determined by electrical impedance expressed as Cell Index. Stem-like HMLE and non-stem-like cells both have significantly reduced attachment when treated with the microtubule polymerization inhibitor colchicine (50µM). For all reattachment assay: lines, mean for quadruplicate wells; bars, SD; representative graph from three independent experiments is shown. (B) Phase contrast images of HMLE non-stem-like (a-d) and stem-like (e-h) subpopulations reattaching from suspension. Panels, 10x magnification; Insets, 60x magnification (Data and figures adapted from Charpentier MS, et al., 2014).
Key Benefits of Using xCELLigence to Study Cell Adhesion/Spreading:
- Real-time monitoring of cell adhesion and spreading.
- Label-free assay requires no fixation, staining or any other sample processing.
- Direct, sensitive, and quantitative.
- Easy quantification of the kinetics of adhesion and spreading.
- Rapid optimization of cell density and extracellular matrix coating conditions.
|xCELLigence Research Grant|
The research grant winner will be provided access to the xCELLigence Real Time Cell Analysis (RTCA) DP instrument, consumables, and consultation for up to 6 months. Apply by December 15, 2019.