Applied Science – Environmental Science

Tim Howell

As awareness of the need for environmental protection has grown globally, cost effective, highly sensitive, and time saving assays for characterizing environmental samples have become a necessity in a broad range of disciplines. Flow cytometry is a powerful tool for environmental studies spanning everything from ecology and zoology to oceanography and microbiology. ACEA’s NovoCyte® flow cytometer is an ideal tool for accurately and rapidly analyzing samples within these sub-disciplines, as detailed below:

Water Quality

Rapid detection of bacteria in aquatic environments has been a challenging task in microbiology studies, especially for natural water containing complex microbial populations. The difficulties are further compounded by examining the activity, size and physical properties of individual populations. ACEA NovoCyte® flow cytometer can detect very small particles with high sensitivity. Combining multi-parameter analytic capability and convenient fluidic maintenance, the NovoCyte® flow cytometer can be easily applied to a variety of microbial studies.

Supporting Information for Marine Biology:

  • Publications:
  1. Early Determination of Toxicant Concentration in Water Supply Using MHE. F. Ibrahim, B. Huang, J. Xing, S. Gabos (Water Research). 2010
Marine Biology

Flow cytometry has routinely been used to study phytoplankton by taking advantage of innate fluorescence molecules that distinguish different species and their physiological conditions. Recent advances in cytometer design and functionality have modernized certain aspects of marine biology applications, creating a more accurate, data-rich, and timely assessment of microscopic marine organisms. Utilizing the two elements of ACEA Biosciences’ NovoCyte® benchtop flow cytometer, volumetric sample delivery via precise syringe injection and extended fluorescence profiling with three excitation lasers, the distribution and abundance of the phytoplankton, bacterial, and viral fractions of seawater can be determined.

Supportive Information on Marine Biology:

  • Publications:
  1. Respiratory Kinetics of Marine Bacteria Exposed to Decreasing Oxygen Concentrations. Gong X, Garcia-Robledo E, Schramm A, Revsbech NP. Appl Environ Microbiol. 2015 Dec 18;82(5):1412-22.
  2. A CRISPR/Cas9 System Adapted for Gene Editing in Marine Algae. Nymark M, Sharma AK, Sparstad T, Bones AM, Winge P. Sci Rep. 2016 Apr 25;6:24951.
  3. Antiproliferative Scalarane-Based Metabolites from the Red Sea Sponge Hyrtios Erectus. Elhady SS, Al-Abd AM, El-Halawany AM, Alahdal AM7 Hassanean HA, Ahmed SA. Mar Drugs. 2016 Jul 8;14(7):130.
  4. Effect of Captivity and Cryopreservation on ROS Production in Solea Senegalensis Spermatozoa. Valcarce DG, Robles V. Reproduction. 2016 Nov;152(5):439-46