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Virus Mediated Cytopathogenicity

Virus-mediated CPE can be quantitatively monitored using the xCELLigence System. In this experiment, Vero cells were mixed with serially diluted West Nile Virus particles, and then seeded in the wells of the E-Plate 96. The virus-mediated CPE was continuously monitored in real time over 6 days on the RTCA SP Instrument. The time and viral titer dependent CPE were automatically recorded. This one-step viral CPE assay format can also be used for viral neutralizing antibody and antiviral agent detection and quantification.

• One-step assay format
• Single-well titration
• 96-well microtiter plate format assay
• Automatic data recording