Real-time Monitoring of West Nile Virus (WNV) Induced CPE on Vero Cells
(A)Normalized cell index plotted as a function of time in hours post infection for wells inoculated with diff erent plaque forming units (PFU) of virus and control (ctrl) wells without virus addition. The horizontal line depicts the 50% point in the decline of the Cell Index(CI), defi ned here as the CIT50 value. (B)CIT50 values were regressed as a function infectious dose in log10 PFU. (Data and figures adapted from Fang Y., et. al., 2011).
Quantitative Detection of WNV Neutralizing Antibody Titer Using Real-Time Monitoring of Virus-Induced CPE
(A)Virus-induced CPE kinetic pattern of Vero cells presented with 106 PFU of WNV incubated with diff erent titers of neutralizing antibody; control (ctrl), no infection. The horizontal line indicates the 50% decline of CI value. (B)Linear regression between CIT50 value and the neutralizing antibody titer. (Data and figures adapted from Fang Y., et. al., 2011).
SELECT Publications for Virus Mediated CPE
1.Real-time cell analysis--a new method for dynamic, quantitative measurement of infectious viruses and antiserum neutralizing activity
Teng Z, Kuang X, Wang J, Zhang X..
J Virol Methods. 2013 Nov;193(2):364-70. doi: 10.1016/j.jviromet.2013.06.034.
2.Novel, real-time cell analysis for measuring viral cytopathogenesis and the efficacy of neutralizing antibodies to the 2009 influenza A (H1N1) virus.
Tian, D., Zhang, W., He, J., Liu, Y., Song, Z., Zhou, Z., Zheng, M., et al.
PloS One. 2012;7(2), e31965. doi:10.1371/journal.pone.0031965.
3.Real-time monitoring of flavivirus induced cytopathogenesis using cell electric impedance technology.
Fang, Y., Ye, P., Wang, X., Xu, X., & Reisen, W.
Journal of virological methods. 2011;173(2), 251–8. doi:10.1016/j.jviromet.2011.02.013.
• A simple alternative method to plaque test for measuring lytic activity of viruses.
• Provide quantitative information about the onset and kinetics of viral mediated cytopathic effects (CPE).
• Rapidly identify the optimal viral titer and assay time point for subsequent screening of inhibitory compounds, neutralizing antibodies and neutralizing serums.