Figure A: Real-time monitoring of HT1080 cell migration using the xCELLigence System with the CIM-Plate16.A serial dilution of 80 000 (magenta line), 40 000 (blue line), 20 000 (green line), 10 000 (brown line), 5 000 (dark blue line) and 2 500 (violet line) cells were seeded in a CIM-Plate 16, and the migration behavior of each was monitored for 24 hours. Cells maintained in serum-free media served as a control (black line).
Figure B: Continuous monitoring of HT1080 cell migration using a fibronectin-coated CIM-Plate 16 with 10% serum serving as the chemoattractant in the lower chamber.Cells in fibronectin-coated wells (blue line) show enhanced migration compared to cells grown without fibronectin-coating (red line). Cells maintained in serum-free media served as a control (magenta and turquoise lines).
Advantages when using the xCELLigence CIM-Plate:
• Monitor cell invasion and migration continuously in real-time
• Receive data free of labeling artifacts using label-free measurements
• Enjoy the full flexibility of the RTCA DP Instrument: Use the E-Plate 16 and CIM-Plate 16 in parallel