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Cell-mediated Cytotoxicity

Figure A:Dynamic monitoring of cell-mediated cytotoxicity. In this example, A549 cancer cells were
seeded in the wells of the E-Plate and allowed to proliferate to about 45 hours, at which
point natural killer cells, NK-92 were added at different densities. The xCELLigence System
can clearly monitor the density-dependent cytotoxicity of NK-92 cells on A549 cells.

Figure B:Dynamic monitoring of antibody-dependent cell-mediated cytotoxicity (ADCC) assays. In this example, cells expressing a specific receptor of interest were seeded in the wells of E-Plate. At the indicated time point PBMCs were added in the absence or presence of different doses of an antibody specific for the receptor of interest. The data clearly shows that the antibody mediates PBMC cytotoxicity upon the target cells.

Advantages
• No labeling needed
• Homogeneous and quantitative assay
• Cytolytic kinetics