Figure 1: Real-time monitoring of cell viability using the xCELLigence System.A) HeLa cells seeded in the E-Plate 96 for 24 hours were treated with different compounds: MG-132 (3.3 m M), doxorubicin (0.33 m M), vincristine (6.2 nM) and 5-FU (11.1 m M). Cell cultures not treated with compound were used as control. Cell Index values were monitored every 15 minutes for 64 hours. The average of duplicate samples is shown; error bars represent the standard deviation of the mean. B) At the end of the experiment (shown in panel A), 64 hours post compound addition, cells were stained with the Cell Proliferation Reagent WST-1. Both CI values and WST-1 findings are shown as fold changes relative to controls. The average of duplicate experiments is shown; error bars represent the standard deviation of the mean.
Figure 2: Real-time monitoring of apoptosis using the xCELLigence System. HeLa cells were treated with various concentrations of the proteasome inhibitor MG132 (panels A & C) and DNA-damaging agent 5-FU (panels B & D). Cell Index values were monitored continuously for 64 hours (panels A & B). Parallel experiments were performed in E-Plates 96 and samples harvested at 16 and 64 hours post compound addition for apoptosis assays (panels C & D). Apoptosis was determined using the Cell Death Detection ELISA Plus kit and expressed in terms of the fold change relative to untreated cell cultures.
• Built-in cell quality control
• Determine the optimal point of compound treatmen
• Real-time monitor cell viability and cytotoxicity
• Determine the optimal time point for end-point assays
• Compound-specific profiling