RT-CIM™ System is a novel system to monitor cell invasion and cell migration. The core of the system is the RT-CIM™ device, composed of an upper chamber (or a top chamber) and a lower chamber (or a bottom chamber). The upper chamber has 16 wells that are sealed at the bottom with a micro-pore-containing polycarbonate (PC) or polyester (PET) membrane. The membrane contains microelectronic sensor arrays that are integrated on its bottom surface. The lower chamber serves as a reservoir for media. Cells are added to the upper chamber and are being detected by the measuring the electronic impedance of sensor electrodes integrated on the membrane after they migrate/invade through pores on the membranes. Based on measured impedance, a dimensionless parameter, Cell Index, is derived and reported to provide quantitative information about number of cells migrated through pores on the micro-porous membranes. Similar to the RT-CES® system, detection of cell invasion and migration on RT-CIM™ system does not require labels or reporters.