The 16X RT-CIM™ System is composed of four main components, W200 RT-CES® analyzer, 16X RT-CIM™ station, a computer which runs RT-CES® SP Software and 16X RT-CIM™ devices. The core of the system is the RT-CIM™ device, composed of an upper chamber (or a top chamber) and a lower chamber (or a bottom chamber). The upper chamber has 16 wells that are sealed at the bottom with a micro-pore-containing polycarbonate (PC) or polyester (PET) membrane. The membrane contains microelectronic sensor arrays that are integrated on its bottom surface. The lower chamber serves as a reservoir for media. Cells are added to the upper chamber, would migrate or invade through pores on the membranes, and arrive on the bottom surface of the membrane and are being detected by the microelectrodes on the membrane. During an assay, the RT-CIM™ devices with the cells added to the top chamber are mounted to the Rt-CIM station, which is located inside a tissue culture incubator. Under the SP Software control, RT-CES® analyzer in conjunction with RT-CIM™ station n automatically select individual sensor electrode arrays for measurement and continuously transfer measured impedance data to the computer. Cell index values, derived from the measured impedances, are continuously displayed on the Software user-interface.