Dynamic monitoring of adherent cell proliferation. In this example, the cancer cell H460 line was seeded at two different cell densities (2500 cells/well and 10,000 cells/well). The cell growth curves were automatically recorded on the RT-CES® system in real time (figure A). From the data, the doubling time of Cell Index, can be calculated, which may indicate the cell doubling time (figure B). Data from RT-CES® system are consistent to that from ATP content detection (figure C).